mRNAs for microtubule proteins are specifically colocalized during the sequential formation of basal body, flagella, and cytoskeletal microtubules in the differentiation of Naegleria gruberi.
mRNAs for microtubule proteins are specifically colocalized during the sequential formation of basal body, flagella, and cytoskeletal microtubules in the differentiation of Naegleria gruberi.
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DOI：
10.1083/jcb.137.4.871
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发表时间：
1997-05-19
影响因子：
7.8
通讯作者：
Lee, J
中科院分区：
文献类型：
--
作者：
Han, JW;Park, JH;Kim, M;Lee, J
我们用原位杂交的方法研究了α-微管蛋白、β-微管蛋白、鞭毛钙调蛋白和第I类基因在鹅观草阿米巴分化为鞭毛过程中的分布。四个α中的三个--微管蛋白、β-微管蛋白和第I类信使核糖核酸--一旦各自基因的转录被激活，在观察到任何微管结构之前，就开始共存于细胞的外围。在分化开始后70min，这些mRNAs被重新定位到生长鞭毛的底部，毗邻细胞骨架微管的基底层和微管组织中心。在另外的15min内，mRNAs被转移到鞭毛细胞的后部，在分化结束时(120min)，观察到非常低水平的mRNAs。细胞松弛素D抑制了mRNAs的阶段特异性定位，表明RNA的定位是肌动蛋白依赖的。由于细胞松弛素D也阻止分化，这增加了肌动蛋白依赖的RNA运动是分化的关键过程的可能性。
We have examined the distribution of four mRNAs—α-tubulin, β-tubulin, flagellar calmodulin, and Class I mRNA—during differentiation of Naegleria gruberi amebas into flagellates by in situ hybridization. Three of the four mRNAs—α-tubulin, β-tubulin, and Class I mRNA—began to be colocalized at the periphery of the cells as soon as transcription of the respective genes was activated and before any microtubular structures were observable. At 70 min after the initiation of differentiation, these mRNAs were relocalized to the base of the growing flagella, adjacent to the basal bodies and microtubule organizing center for the cytoskeletal microtubules. Within an additional 15 min, the mRNAs were translocated to the posterior of the flagellated cells, and by the end of differentiation (120 min), very low levels of the mRNAs were observed. Cytochalasin D inhibited stage-specific localization of the mRNAs, demonstrating that RNA localization was actin dependent. Since cytochalasin D also blocked differentiation, this raises the possibility that actin-dependent RNA movement is an essential process for differentiation.
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Naegleria鞭毛中的两个钙调蛋白：在分化过程中对mRNA丰度的表征，细胞内分离和编程的调节。
影响因子：
7.8
作者：
FULTON, C;CHEN, KL;LAI, EY
通讯作者：
LAI, EY
影响因子：
4.8
作者：
FULTON, C;LAI, EY;REMILLARD, SP
通讯作者：
REMILLARD, SP
影响因子：
7.8
作者：
SHEA, DK;WALSH, CJ
通讯作者：
WALSH, CJ
影响因子：
7.8
作者：
BARON, AT;SALISBURY, JL
通讯作者：
SALISBURY, JL
影响因子：
5.3
作者：
LEE, JH;WALSH, CJ
通讯作者：
WALSH, CJ