Shotgun sequencing of Yersinia enterocolitica strain W22703 (biotype 2, serotype O:9): genomic evidence for oscillation between invertebrates and mammals.
Shotgun sequencing of Yersinia enterocolitica strain W22703 (biotype 2, serotype O:9): genomic evidence for oscillation between invertebrates and mammals.
复制标题
DOI：
10.1186/1471-2164-12-168
复制
发表时间：
2011-03-31
期刊：
影响因子：
4.4
通讯作者：
Rattei T
中科院分区：
文献类型：
--
作者：
Fuchs TM;Brandt K;Starke M;Rattei T
引起人类轻度胃肠炎的小肠结肠炎耶尔森菌菌株在代谢和毒力特性方面非常不同。菌株W22703(生物型2，血清型O：9)最近被鉴定为具有杀线虫和杀虫活性。为了更好地了解对昆虫和人类的致病性之间的关系，我们将W22703基因组与迄今已测序的唯一一株小肠结肠炎耶尔森菌高致病性菌株8081(生物型1B；血清型O：8)的基因组进行了比较。我们使用全基因组猎枪数据对菌株W22703的序列进行了组装、注释和分析。许多与肠道存活和致病有关的因素被认为是8081和W22703基因组所共有的，其中渗透调节的周质葡聚糖、氢酶、钴胺依赖途径、铁摄取系统和参与紧密黏附的耶尔森氏菌基因组岛1(YGI-1)是共同的。然而，与另一种毒株相比，约550组基因显示出对每一种毒株的特异性。W22703基因组142kb的可塑性区(PZ)携带一个约40kb的古老鞭毛簇Flg-2，但它缺乏8081PZ的致病岛YapiYe、分泌系统ysa和yts1以及其他毒力决定因素。它的组成突出了该基因组区域的显著变异性，并表明它对生物型1B菌株相对于W22703具有更高的致病性做出了贡献。在W22703的基因组中发现了一种新的三型嵌合结构分泌系统，这是人类致病耶尔森氏菌基因组中所没有的，而在非致病耶尔森氏菌基因组中是保守的。我们确定了W22703的几个差异区域，这些区域主要编码转运蛋白、调节因子、代谢途径和防御因子。W22703序列分析揭示了一种与其他致病性小肠结肠炎耶尔森菌不同的基因组组成，从而为小肠结肠炎耶尔森氏菌泛基因组提供了新的数据。这项研究还进一步阐明了该病原体应对其环境的策略。
Yersinia enterocolitica strains responsible for mild gastroenteritis in humans are very diverse with respect to their metabolic and virulence properties. Strain W22703 (biotype 2, serotype O:9) was recently identified to possess nematocidal and insecticidal activity. To better understand the relationship between pathogenicity towards insects and humans, we compared the W22703 genome with that of the highly pathogenic strain 8081 (biotype1B; serotype O:8), the only Y. enterocolitica strain sequenced so far. We used whole-genome shotgun data to assemble, annotate and analyse the sequence of strain W22703. Numerous factors assumed to contribute to enteric survival and pathogenesis, among them osmoregulated periplasmic glucan, hydrogenases, cobalamin-dependent pathways, iron uptake systems and the Yersinia genome island 1 (YGI-1) involved in tight adherence were identified to be common to the 8081 and W22703 genomes. However, sets of ~550 genes revealed to be specific for each of them in comparison to the other strain. The plasticity zone (PZ) of 142 kb in the W22703 genome carries an ancient flagellar cluster Flg-2 of ~40 kb, but it lacks the pathogenicity island YAPIYe, the secretion system ysa and yts1, and other virulence determinants of the 8081 PZ. Its composition underlines the prominent variability of this genome region and demonstrates its contribution to the higher pathogenicity of biotype 1B strains with respect to W22703. A novel type three secretion system of mosaic structure was found in the genome of W22703 that is absent in the sequenced strains of the human pathogenic Yersinia species, but conserved in the genomes of the apathogenic species. We identified several regions of differences in W22703 that mainly code for transporters, regulators, metabolic pathways, and defence factors. The W22703 sequence analysis revealed a genome composition distinct from other pathogenic Yersinia enterocolitica strains, thus contributing novel data to the Y. enterocolitica pan-genome. This study also sheds further light on the strategies of this pathogen to cope with its environments.
登录
查看更多内容
影响因子：
4.4
作者：
Heermann R;Fuchs TM
通讯作者：
Fuchs TM
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
3.2
作者：
Bresolin, G;Neuhaus, K;Fuchs, TM
通讯作者：
Fuchs, TM
DOI：
10.1099/00221287-87-2-285
发表时间：
1975-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
CORNELIS, G;COLSON, C
通讯作者：
COLSON, C
影响因子：
14.9
作者：
Besemer, J;Lomsadze, A;Borodovsky, M
通讯作者：
Borodovsky, M