Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP.
Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP.
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DOI：
10.1186/1756-3305-6-227
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发表时间：
2013-08-07
影响因子：
3.2
通讯作者：
Zhu XQ
中科院分区：
文献类型：
--
作者：
Jiang HH;Huang SY;Zhou DH;Zhang XX;Su C;Deng SZ;Zhu XQ
刚地弓形虫是一种广泛流行的原生动物寄生虫，在人类和动物中引起严重的弓形虫病。采用多位点聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对江西、四川、广东和重庆猪源弓形虫分离株的遗传多样性进行了研究。从疑似弓形虫病猪肺门淋巴结提取38份DNA样本，采用B1基因半巢式PCR检测弓形虫的存在。阳性DNA样本在11个遗传标记上分型，包括10个核位点，即SAG1、5′-SAG2和3′-SAG2，替代SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和顶质体位点Apico。38份DNA样本中有25份呈弓形虫B1基因阳性。25份样本中有17份可获得所有基因座的完整基因分型数据。结果显示两种基因型（弓形虫PCR-RFLP基因型#9和#3）。16份样本属于基因型#9，这是中国大陆的主要谱系，1份样本属于基因型#3，这是II型变异。据我们所知，这是江西省、四川省和重庆市猪源弓形虫分离株遗传分型的首次报道，也是弓形虫3t型的首次报道。刚地虫来自中国的猪。这些结果对人类食源性弓形虫病的预防和控制具有指导意义。
Toxoplasma gondii is a widely prevalent protozoan parasite that causes serious toxoplasmosis in humans and animals. The present study aimed to determine the genetic diversity of T. gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China using multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. A total of 38 DNA samples were extracted from hilar lymph nodes of pigs with suspected toxoplasmosis, and were detected for the presence of T. gondii by semi-nested PCR of B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci, namely, SAG1, 5′-SAG2 and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast locus Apico. Twenty-five of the 38 DNA samples were T. gondii B1 gene positive. Complete genotyping data for all loci could be obtained for 17 of the 25 samples. Two genotypes were revealed (ToxoDB PCR-RFLP genotypes #9 and #3). Sixteen samples belong to genotype #9 which is the major lineage in mainland China and one sample belongs to genotype #3 which is Type II variant. To our knowledge, this is the first report of genetic typing of T. gondii isolates from pigs in Jiangxi, Sichuan Province and Chongqing Municipality, and the first report of ToxoDB #3 T. gondii from pigs in China. These results have implications for the prevention and control of foodborne toxoplasmosis in humans.
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