A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
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DOI：
10.1016/j.cell.2012.02.055
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发表时间：
2012-04-27
期刊：
影响因子：
64.5
通讯作者：
Losick R
中科院分区：
文献类型：
--
作者：
Kolodkin-Gal I;Cao S;Chai L;Böttcher T;Kolter R;Clardy J;Losick R
生物膜是由蛋白质和胞外多糖组成的细胞外基质将细菌聚集在一起的结构化群落。生物膜的寿命通常是有限的，随着营养物质的耗尽和废物的积累而分解。D-氨基酸以前被认为是一种自身产生的因子，通过导致枯草芽孢杆菌基质中蛋白质成分的释放来介导生物膜的分解。在这里，我们报告了枯草杆菌产生一种额外的生物膜分解因子，去甲精胺。动态光散射和扫描电子显微镜实验表明去甲精胺与胞外多糖直接、特异地相互作用。D-氨基酸和去甲精胺共同作用，破坏现有的生物膜，而被阻止生产这两种生物膜的突变体都形成了长寿命的生物膜。去甲硝咪胺，但不是密切相关的多胺，可以防止枯草杆菌、大肠杆菌和金黄色葡萄球菌生物膜的形成。
Biofilms are structured communities of bacteria that are held together by an extracellular matrix consisting of protein and exopolysaccharide. Biofilms often have a limited lifespan, disassembling as nutrients become exhausted and waste products accumulate. D-amino acids were previously identified as a self-produced factor that mediates biofilm disassembly by causing the release of the protein component of the matrix in Bacillus subtilis. Here we report that B. subtilis produces an additional biofilm-disassembly factor, norspermidine. Dynamic light scattering and scanning electron microscopy experiments indicated that norspermidine interacts directly and specifically with exopolysaccharide. D-amino acids and norspermidine acted together to break down existing biofilms and mutants blocked in the production of both formed long-lived biofilms. Norspermidine, but not closely related polyamines, prevented biofilm formation by B. subtilis, Escherichia coli and Staphylococcus aureus.
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影响因子：
3.2
作者：
Branda, SS;González-Pastor, JE;Kolter, R
通讯作者：
Kolter, R
影响因子：
4.4
作者：
McSwain, BS;Irvine, RL;Wilderer, PA
通讯作者：
Wilderer, PA
DOI：
10.1073/pnas.0910560107
发表时间：
2010-02-02
影响因子：
11.1
作者：
Romero, Diego;Aguilar, Claudio;Kolter, Roberto
通讯作者：
Kolter, Roberto
影响因子：
3.2
作者：
Danese, PN;Pratt, LA;Kolter, R
通讯作者：
Kolter, R
影响因子：
11.4
作者：
Cava, Felipe;de Pedro, Miguel A.;Waldor, Matthew K.
通讯作者：
Waldor, Matthew K.