UV-B promotes flavonoid biosynthesis in Ginkgo biloba by inducing the GbHY5-GbMYB1-GbFLS module.
UV-B promotes flavonoid biosynthesis in Ginkgo biloba by inducing the GbHY5-GbMYB1-GbFLS module.
复制标题
DOI：
10.1093/hr/uhad118
复制
发表时间：
2023-08
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
银杏（Ginkgo biloba）叶具有药用价值，因为它们含有大量的次生代谢产物，如黄酮类化合物。研究发现，银杏叶中黄酮类化合物含量在高海拔地区（青藏高原）显著增加。针对青藏高原高UV-B辐射的主要环境特征，对银杏幼苗进行模拟UV-B辐射处理，结果表明，UV-B辐射处理后银杏叶片中黄酮类化合物含量显著增加。结合我们以前的研究结果，我们确定转录因子GbHY 5可能在UV-B辐射响应中起关键作用。过量表达GbHY 5显著促进银杏愈伤组织和拟南芥中黄酮类化合物的积累。酵母双杂交和实时荧光定量PCR结果表明，GbHY 5通过与GbMYB 1蛋白相互作用促进GbMYB 1的表达。GbMYB 1在银杏愈伤组织和A.拟南芥还显著促进类黄酮的生物合成。GbFLS编码类黄酮生物合成中的关键酶，其启动子具有GbHY 5和GbMYB 1的结合元件。双荧光素酶报告基因检测表明，虽然GbHY 5和GbMYB 1单独激活了GbFLS的表达，但它们的共表达实现了更大的激活。我们的分析揭示了UV-B诱导的GbHY 5-GbMYB 1-GbFLS模块促进银杏类黄酮生物合成的分子机制，并为利用UV-B辐射提高银杏叶类黄酮含量提供了见解。
Ginkgo biloba (ginkgo) leaves have medicinal value due to their high levels of secondary metabolites, such as flavonoids. We found that the flavonoid content in ginkgo leaves increases significantly at high altitudes (Qinghai-Tibet Plateau). Considering that high UV-B radiation is among the key environmental characteristics of the Qinghai-Tibet Plateau, we carried out simulated UV-B treatments on ginkgo seedlings and found that the flavonoid content of the leaves increased significantly following the treatments. Combined with results from our previous studies, we determined that the transcription factor GbHY5 may play a key role in responses to UV-B radiation. Overexpression of GbHY5 significantly promoted the accumulation of flavonoids in both ginkgo callus and Arabidopsis thaliana. Furthermore, yeast two-hybrid and real-time quantitative PCR showed that GbHY5 promoted the expression of GbMYB1 by interacting with GbMYB1 protein. Overexpression of GbMYB1 in ginkgo callus and A. thaliana also significantly promoted flavonoid biosynthesis. GbFLS encodes a key enzyme in flavonoid biosynthesis, and its promoter has binding elements of GbHY5 and GbMYB1. A dual-luciferase reporter assay indicated that while GbHY5 and GbMYB1 activated the expression of GbFLS individually, their co-expression achieved greater activation. Our analyses reveal the molecular mechanisms by which the UV-B-induced GbHY5-GbMYB1-GbFLS module promotes flavonoid biosynthesis in ginkgo, and they provide insight into the use of UV-B radiation to enhance the flavonoid content of ginkgo leaves.
登录
查看更多内容
影响因子：
3.1
作者：
Aksakal, Ozkan;Tabay, Dilruba;Esim, Nevzat
通讯作者：
Esim, Nevzat
影响因子：
8.7
作者：
Li T;Yamane H;Tao R
通讯作者：
Tao R
影响因子：
5.6
作者：
Karagiannis E;Tanou G;Samiotaki M;Michailidis M;Diamantidis G;Minas IS;Molassiotis A
通讯作者：
Molassiotis A
影响因子：
2.9
作者：
Chen, Ke-ji
通讯作者：
Chen, Ke-ji
影响因子：
5.9
作者：
Liu, Sian;Meng, Zhaolong;Wang, Li
通讯作者：
Wang, Li