Identification of viral pathogen diversity in sewage sludge by metagenome analysis.
Identification of viral pathogen diversity in sewage sludge by metagenome analysis.
复制标题
DOI：
10.1021/es305181x
复制
发表时间：
2013-02-19
影响因子：
11.4
通讯作者：
Peccia J
中科院分区：
文献类型：
--
作者：
Bibby K;Peccia J
存在于人群中的大量病毒可能被排泄到污水收集系统中，并集中在污水污泥中。在美国，经处理的污水污泥（B类生物固体）的主要归宿是作为土壤改良剂施用于农业用地。为了表征和理解与土地应用相关的感染风险，并描述人群中病毒的多样性，将鸟枪病毒宏基因组学应用于来自美国大陆5个污水处理厂的10个污水污泥样品，每个样品服务于10万至100万人。生产和组装了近3.3亿个DNA序列，注释导致在污水污泥中识别出43种不同类型的人类病毒（26种DNA，17种RNA）。新的见解包括新出现的病毒（如冠状病毒HKU1，Klassevirus和Cosavirus）的高丰度，呼吸道病毒的强代表性，以及肠道病毒的相对较小的丰度和发生率。病毒宏基因组序列注释是可重复的，并且对选定病毒的基于PCR的独立鉴定表明病毒宏基因组是对真实病毒发生和多样性的保守估计。这些结果代表了迄今为止任何废水样品中人类病毒多样性的最完整描述，为工程师和环境科学家提供了关于重要病毒因子和暴露于废水和污水污泥的感染途径的关键信息，并代表了理解B类生物固体病原体含量的重大飞跃。
The large diversity of viruses that exist in human populations are potentially excreted into sewage collection systems and concentrated in sewage sludge. In the US, the primary fate of processed sewage sludge (class B biosolids) is application to agricultural land as a soil amendment. To characterize and understand infectious risks associated with land application, and to describe the diversity of viruses in human populations, shotgun viral metagenomics was applied to 10 sewage sludge samples from 5 wastewater treatment plants throughout the continental U.S, each serving between 100,000 and 1,000,000 people. Nearly 330 million DNA sequences were produced and assembled, and annotation resulted in identifying 43 (26 DNA, 17 RNA) different types of human viruses in sewage sludge. Novel insights include the high abundance of newly emerging viruses (e.g. Coronavirus HKU1, Klassevirus, and Cosavirus) the strong representation of respiratory viruses, and the relatively minor abundance and occurrence of Enteroviruses. Viral metagenome sequence annotations were reproducible and independent PCR-based identification of selected viruses suggests that viral metagenomes were a conservative estimate of the true viral occurrence and diversity. These results represent the most complete description of human virus diversity in any wastewater sample to date, provide engineers and environmental scientists with critical information on important viral agents and routes of infection from exposure to wastewater and sewage sludge, and represent a significant leap forward in understanding the pathogen content of class B biosolids.
登录
查看更多内容
影响因子：
4.4
作者：
McLennan, S. Devin;Peterson, Lauren A.;Rose, Joan B.
通讯作者：
Rose, Joan B.
影响因子：
4.4
作者：
Gerba, CP;Gramos, DM;Nwachuku, N
通讯作者：
Nwachuku, N
影响因子：
4.4
作者：
Wong, Kelvin;Onan, Brandon M.;Xagoraraki, Irene
通讯作者：
Xagoraraki, Irene
影响因子：
4.8
作者：
Greninger, Alexander L.;Runckel, Charles;DeRisi, Joseph L.
通讯作者：
DeRisi, Joseph L.
未经处理的污水含有多种病毒种群。
影响因子：
6.4
作者：
Cantalupo PG;Calgua B;Zhao G;Hundesa A;Wier AD;Katz JP;Grabe M;Hendrix RW;Girones R;Wang D;Pipas JM
通讯作者：
Pipas JM