Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos.
Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos.
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DOI：
10.1016/j.aca.2011.03.011
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发表时间：
2011-05-05
影响因子：
6.2
通讯作者：
Lee HS
中科院分区：
文献类型：
--
作者：
Kim YA;Lee EH;Kim KO;Lee YT;Hammock BD;Lee HS
开发了一种基于竞争性抗原包被形式、使用胶体金作为标记的免疫层析测定 (ICA)，用于检测有机磷杀虫剂毒死蜱。 ICA 测试条由带有检测区的膜、样品垫和吸收垫组成。膜分别涂有毒死蜱半抗原-OVA缀合物（测试线）和抗小鼠IgG（对照线）。基于样品中的迁移分析物与测试条上固定的分析物半抗原之间对测试条上迁移的抗体胶体金（Ab-CG）缀合物的结合位点进行竞争的事实，本研究表明两种迁移物质之间的相对迁移速度是竞争性ICA灵敏检测的一个至关重要的因素。该标准用于确认硝化纤维素 (NC) 膜对于毒死蜱 ICA 的适用性。毒死蜱标准品和农业样品中添加的毒死蜱的 ICA 检出限分别为 10 ng mL−1 和 50 ng mL−1。 ICA测试测定时间小于10分钟，适合毒死蜱现场快速检测。
An immunochromatographic assay (ICA) based on competitive antigen-coated format using colloidal gold as the label was developed for the detection of the organophosphorus insecticide chlorpyrifos. The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with chlorpyrifos hapten-OVA conjugate (test line) and anti-mouse IgG (control line). Based on the fact that the competition is between the migrating analyte in the sample and the analyte hapten immobilized on the test strip for the binding sites of the antibody-colloidal gold (Ab-CG) conjugate migrating on the test strip, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. This criterion was utilized for the confirmation of appropriateness of a nitrocellulose (NC) membrane for chlorpyrifos ICA. The detection limit of the ICA for chlorpyrifos standard and chlorpyrifos spiked into agricultural samples were 10 and 50 ng mL−1, respectively. The assay time for the ICA test was less than 10 min, suitable for rapid on-site testing of chlorpyrifos.
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DOI：
10.1007/978-1-59745-240-3_6
发表时间：
2009-01-01
期刊：
LATERAL FLOW IMMUNOASSAY
影响因子：
--
作者：
Mansfield, Michael A.
通讯作者：
Mansfield, Michael A.
影响因子：
11.4
作者：
Kaur, Jasdeep;Singh, K. Vikas;Suri, C. Raman
通讯作者：
Suri, C. Raman
用于高效在线净化拟除虫菊酯并随后进行高效液相色谱分析的类特异性免疫亲和整体材料
DOI：
10.1016/j.jchromb.2009.06.007
发表时间：
2010-01-15
影响因子：
3
作者：
Liang, Yuan;Zhou, Shuang;Liu, Huwei
通讯作者：
Liu, Huwei
DOI：
10.3891/acta.chem.scand.50-0141
发表时间：
1996-02-01
期刊：
ACTA CHEMICA SCANDINAVICA
影响因子：
--
作者：
Laitinen, MPA;Vuento, M
通讯作者：
Vuento, M
影响因子：
6.1
作者：
Chen, Liben;Wang, Zhengfang;Han, Bo
通讯作者：
Han, Bo