Combined taurine, epigallocatechin gallate and genistein therapy reduces HSC-T6 cell proliferation and modulates the expression of fibrogenic factors.
Combined taurine, epigallocatechin gallate and genistein therapy reduces HSC-T6 cell proliferation and modulates the expression of fibrogenic factors.
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牛磺酸、表没食子儿茶素没食子酸酯和金雀异黄素联合治疗可减少 HSC-T6 细胞增殖并调节纤维形成因子的表达。
DOI：
10.3390/ijms141020543
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发表时间：
2013-10-14
影响因子：
5.6
通讯作者：
Liao M
中科院分区：
文献类型：
--
作者：
Li Y;Luo Y;Zhang X;Lin X;He M;Liao M
Hepatic fibrogenesis involves the activation of hepatic stellate cells (HSCs), which synthesize excess extracellular matrix and contribute to the development of liver fibrosis. In a prior study we tested the effect of combined treatment with taurine, epigallocatechin gallate and genistein on the development of alcohol-induced liver fibrosis in vitro. In this study, the biological activity of the combination of these molecules was assessed by measuring its effect on cell proliferation, fibrosis-related gene expression, and proteomic expression profiling in the activated HSC cell line, HSC-T6. HSC-T6 cells were incubated with different concentrations of the drug combination taurine, epigallocatechin gallate and genistein. Cell proliferation was evaluated by MTT assay. Transforming growth factor β1 (TGF-β1), collagen type I (Col-I), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2) mRNA were analyzed by semi-quantitative reverse-transcription PCR. Proteomic profiling of HSC-T6 cells was also performed by SELDI-TOF-MS. Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased. A total of 176 protein m/z peaks were identified. The intensities of 10 protein peaks were downregulated and two protein peaks were upregulated in HSC-T6 cells after combined drug treatment. In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression. The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes.
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影响因子：
7.3
作者：
Madhani, M;Scotland, RS;Hobbs, AJ
通讯作者：
Hobbs, AJ
影响因子：
3.2
作者：
Lee, Ursula E.;Friedman, Scott L.
通讯作者：
Friedman, Scott L.
影响因子：
25.7
作者：
Miyazaki, T;Karube, M;Bouscarel, B
通讯作者：
Bouscarel, B
影响因子：
4.1
作者：
Al-Gubory, KH;Huet, JC;Locatelli, A
通讯作者：
Locatelli, A
影响因子：
4.2
作者：
Kato, J;Ido, A;Tsubouchi, H
通讯作者：
Tsubouchi, H