Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis.
Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis.
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DOI：
10.1038/s41598-021-93345-y
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发表时间：
2021-07-06
影响因子：
4.6
通讯作者：
Kiyono H
中科院分区：
文献类型：
--
作者：
Mizutani T;Aboagye SY;Ishizaka A;Afum T;Mensah GI;Asante-Poku A;Asandem DA;Parbie PK;Abana CZ;Kushitor D;Bonney EY;Adachi M;Hori H;Ishikawa K;Matano T;Taniguchi K;Opare D;Arhin D;Asiedu-Bekoe F;Ampofo WK;Yeboah-Manu D;Koram KA;Anang AK;Kiyono H
在非洲和南亚，与腹泻相关的急性胃肠炎被认为是一种严重的疾病。在这项研究中，我们通过16S rRNA测序对粪便进行微生物组分析，研究了加纳腹泻病原体和相应肠道微生物群的趋势。从2017年到2018年，共有80名腹泻患者和34名健康成年人作为对照参加了这项研究。腹泻患者中，诺如病毒阳性39例，轮状病毒阳性18例。腹泻患者的物种丰富度分析（Chao1）低于对照组。β多样性分析显示两组之间存在显著差异。几种与绦虫有关的病原体（例如，腹泻患者检出志贺菌、克雷伯菌和弯曲菌。此外，与这些病原体和肠道病毒（例如，诺如病毒和轮状病毒）。丹毒丝菌科和葡萄球菌科的水平显着不同诺如病毒阳性和阴性的腹泻粪便，和10个预测的代谢途径，包括碳水化合物代谢途径，轮状病毒阳性腹泻患者和对照组之间的显着差异。这项对加纳腹泻病原体的比较研究揭示了与腹泻相关的肠道微生物群特征的特定趋势，以及病毒性胃肠炎中发生的病原体依赖性生态失调。
Acute gastroenteritis associated with diarrhea is considered a serious disease in Africa and South Asia. In this study, we examined the trends in the causative pathogens of diarrhea and the corresponding gut microbiota in Ghana using microbiome analysis performed on diarrheic stools via 16S rRNA sequencing. In total, 80 patients with diarrhea and 34 healthy adults as controls, from 2017 to 2018, were enrolled in the study. Among the patients with diarrhea, 39 were norovirus-positive and 18 were rotavirus-positive. The analysis of species richness (Chao1) was lower in patients with diarrhea than that in controls. Beta-diversity analysis revealed significant differences between the two groups. Several diarrhea-related pathogens (e.g., Escherichia-Shigella, Klebsiella and Campylobacter) were detected in patients with diarrhea. Furthermore, co-infection with these pathogens and enteroviruses (e.g., norovirus and rotavirus) was observed in several cases. Levels of both Erysipelotrichaceae and Staphylococcaceae family markedly differed between norovirus-positive and -negative diarrheic stools, and the 10 predicted metabolic pathways, including the carbohydrate metabolism pathway, showed significant differences between rotavirus-positive patients with diarrhea and controls. This comparative study of diarrheal pathogens in Ghana revealed specific trends in the gut microbiota signature associated with diarrhea and that pathogen-dependent dysbiosis occurred in viral gastroenteritis.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
3.7
作者：
Chen W;Liu F;Ling Z;Tong X;Xiang C
通讯作者：
Xiang C
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.
影响因子：
4.8
作者：
Kreidieh K;Charide R;Dbaibo G;Melhem NM
通讯作者：
Melhem NM