White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats.
White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats.
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DOI：
10.1038/s41598-018-33975-x
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发表时间：
2018-10-19
影响因子：
4.6
通讯作者：
Misra V
中科院分区：
文献类型：
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作者：
Davy CM;Donaldson ME;Subudhi S;Rapin N;Warnecke L;Turner JM;Bollinger TK;Kyle CJ;Dorville NAS;Kunkel EL;Norquay KJO;Dzal YA;Willis CKR;Misra V
病毒从蝙蝠传播到其他动物可能与它们之间接触的增加以及蝙蝠释放病毒的增加有关。在这里，我们测试的预测，小棕蝙蝠（鼠耳蝠lucifugus）共同感染的M。与仅感染Myl-CoV或仅感染P. destructans的蝙蝠相比，感染Lucifugus冠状病毒（Myl-CoV）和引起蝙蝠白鼻综合征（WNS）的真菌Pseudogymnocleptoms destructans（Pd）的蝙蝠表现出不同的疾病严重程度、病毒脱落和分子反应。我们利用了Myl-CoV在蝙蝠中的自然持久性，这些蝙蝠在先前的研究中实验性地接种了破坏性变形杆菌。在这里，我们表明，病毒感染的蝙蝠肠道也感染了真菌，平均含有60倍以上的病毒RNA比蝙蝠与病毒单独。肠道中病毒RNA的增加与真菌相关病理学的严重程度相关。此外，感染真菌的蝙蝠的肠道表现出不同的有丝分裂原活化蛋白激酶途径和细胞因子相关的转录本的表达，无论病毒的存在。在感染真菌的蝙蝠中，冠状病毒抗体水平也较高。我们的研究结果表明，WNS的全身效应可能下调蝙蝠持续感染M。Lucifugus冠状病毒，并增加病毒脱落的可能性。
Spillover of viruses from bats to other animals may be associated with increased contact between them, as well as increased shedding of viruses by bats. Here, we tested the prediction that little brown bats (Myotis lucifugus) co-infected with the M. lucifugus coronavirus (Myl-CoV) and with Pseudogymnoascus destructans (Pd), the fungus that causes bat white-nose syndrome (WNS), exhibit different disease severity, viral shedding and molecular responses than bats infected with only Myl-CoV or only P. destructans. We took advantage of the natural persistence of Myl-CoV in bats that were experimentally inoculated with P. destructans in a previous study. Here, we show that the intestines of virus-infected bats that were also infected with fungus contained on average 60-fold more viral RNA than bats with virus alone. Increased viral RNA in the intestines correlated with the severity of fungus-related pathology. Additionally, the intestines of bats infected with fungus exhibited different expression of mitogen-activated protein kinase pathway and cytokine related transcripts, irrespective of viral presence. Levels of coronavirus antibodies were also higher in fungal-infected bats. Our results suggest that the systemic effects of WNS may down-regulate anti-viral responses in bats persistently infected with M. lucifugus coronavirus and increase the potential of virus shedding.
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影响因子：
11.8
作者：
Drexler JF;Corman VM;Wegner T;Tateno AF;Zerbinati RM;Gloza-Rausch F;Seebens A;Müller MA;Drosten C
通讯作者：
Drosten C
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
6.4
作者：
Huang YW;Dickerman AW;Piñeyro P;Li L;Fang L;Kiehne R;Opriessnig T;Meng XJ
通讯作者：
Meng XJ
影响因子：
2.6
作者：
Davy CM;Donaldson ME;Willis CKR;Saville BJ;McGuire LP;Mayberry H;Wilcox A;Wibbelt G;Misra V;Bollinger T;Kyle CJ
通讯作者：
Kyle CJ
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y