Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens.
Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens.
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子宫转录组分析揭示了与年轻和老年蛋鸡蛋壳超微结构差异相关的 mRNA 表达变化
DOI：
10.1186/s12864-020-07177-7
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发表时间：
2020-11-09
期刊：
影响因子：
4.4
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Feng J;Zhang HJ;Wu SG;Qi GH;Wang J
研究背景产蛋后期蛋壳质量差会造成经济损失。是蛋制品质量安全的主要威胁。超微结构中的细胞凋亡相关变化被认为是导致这种恶化的原因。产蛋母鸡子宫蛋壳形成是一个高度复杂的过程，受基因和生物学途径的精确调控。本研究通过对蛋壳的物理、力学和超微结构特性的研究，并利用RNA测序技术，分析了产蛋高峰期蛋鸡子宫组织中转录组的差异（年轻的母鸡）和后期结果与青年母鸡组相比，老年母鸡组蛋壳的断裂强度和断裂韧性下降，并伴有乳头层增厚、早期融合率降低等超微结构改变。在蛋壳形成的初期，共有183个差异表达的基因（DEG; 125个上调和58个下调），在后期阶段的蛋鸡子宫相对于那些在高峰生产。与年轻母鸡相比，与抗原加工和呈递相关的基因本体论术语注释的DEG在老年母鸡中下调。促炎细胞因子IL-1β在老龄母鸡子宫中的含量高于青年母鸡。结论产蛋鸡产蛋后期子宫内基质蛋白基因表达的改变，沿着免疫功能的下降，可能是导致蛋壳超微结构和力学性能年龄相关性损伤的重要原因。本研究加深了人们对蛋壳超微结构随年龄变化的认识，并为产蛋后期蛋壳质量的改善提供了潜在的目标。
BackgroundLower eggshell quality in the late laying period leads to economic loss. It is a major threat to the quality and safety of egg products. Age-related variations in ultrastructure were thought to induce this deterioration. Eggshell formation is a highly complex process under precise regulation of genes and biological pathways in uterus of laying hens. Herein, we evaluated the physical, mechanical and ultrastructure properties of eggshell and conducted RNA sequencing to learn the transcriptomic differences in uterus between laying hens in the peak (young hens) and late phase (aged hens) of production.ResultsThe declined breaking strength and fracture toughness of eggshell were observed in aged hen group compared to those in young hen group, accompanied with ultrastructure variations including the increased thickness of mammillary layer and the decreased incidence of early fusion. During the initial stage of eggshell formation, a total of 183 differentially expressed genes (DEGs; 125 upregulated and 58 downregulated) were identified in uterus of laying hens in the late phase in relative to those at peak production. The DEGs annotated to Gene Ontology terms related to antigen processing and presentation were downregulated in aged hens compared to young hens. The contents of proinflammatory cytokine IL-1β in uterus were higher in aged hens relative to those in young hens. Besides, the genes of some matrix proteins potentially involved in eggshell mineralization, such as ovalbumin, versican and glypican 3, were also differentially expressed between two groups.ConclusionsAltered gene expression of matrix proteins along with the compromised immune function in uterus of laying hens in the late phase of production may conduce to age-related impairments of eggshell ultrastructure and mechanical properties. The current study enhances our understanding of the age-related deteriorations in eggshell ultrastructure and provides potential targets for improvement of eggshell quality in the late laying period.
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影响因子：
4.4
作者：
Brionne A;Nys Y;Hennequet-Antier C;Gautron J
通讯作者：
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