Identification and characterization of potential NBS-encoding resistance genes and induction kinetics of a putative candidate gene associated with downy mildew resistance in Cucumis.
Identification and characterization of potential NBS-encoding resistance genes and induction kinetics of a putative candidate gene associated with downy mildew resistance in Cucumis.
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黄瓜中潜在 NBS 编码抗性基因的鉴定和表征以及与霜霉病抗性相关的假定候选基因的诱导动力学
DOI：
10.1186/1471-2229-10-186
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发表时间：
2010-08-23
影响因子：
5.3
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Wan H;Zhao Z;Malik AA;Qian C;Chen J
由于霜霉病菌生理小种的变异和突变，近年来霜霉病已成为世界范围内黄瓜最严重的叶部病害。新的抗霜霉病性已在野生黄瓜中鉴定出来。海斯特里C. hystrix和栽培黄瓜（C. sativusL.），一个渐渗系（IL 5211 S）被鉴定为对霜霉病高度抗性。核苷酸结合位点和富含亮氨酸重复序列（NBS-LRR）基因是植物中克隆到的最大一类具有高度保守结构域的抗病基因，结果根据抗病蛋白NBS结构域的保守序列设计简并引物，从IL 5211 S中分离到NBS-1基因。IL 5211 S的序列。共鉴定出28个序列，命名为黄瓜（Cucumber（C. sativus= CS）抗性基因类似物作为CSRGA。多基因分析将这些序列分为四个不同的类。实时荧光定量PCR（qRT-PCR）分析表明，这些CSRGA在叶、根和茎中均有不同程度的表达。此外，C. hystrix诱导的部分CSRGA在栽培黄瓜中的表达，特别是CSRGA 23，与回交亲本CC 3相比增加了四倍。此外，在P.还在不同时间点分析了古巴病菌感染和非生物胁迫。结果表明，P. Cubensistreatment和四种测试的非生物刺激物，MeJA，SA，阿坝和H2 O2，在接种72 h内触发了CSRGA 23的显著诱导。结果表明，CSRGA 23可能在黄瓜抗磷胁迫中发挥重要作用。结论从IL 5211 S中成功分离出4类NBS型RGAs，CSRGA 23可能参与了对黄瓜变形杆菌的主动防御反应。cubensis被证明。这些结果将有助于开发与霜霉病抗性相关的类似分子标记，并进一步阐明IL 5211 S的抗病分子机制。
BackgroundDue to the variation and mutation of the races ofPseudoperonospora cubensis, downy mildew has in recent years become the most devastating leaf disease of cucumber worldwide. Novel resistance to downy mildew has been identified in the wildCucumisspecies,C. hystrixChakr. After the successful hybridization betweenC. hystrixand cultivated cucumber (C. sativusL.), an introgression line (IL5211S) was identified as highly resistant to downy mildew. Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes are the largest class of disease resistance genes cloned from plant with highly conserved domains, which can be used to facilitate the isolation of candidate genes associated with downy mildew resistance in IL5211S.ResultsDegenerate primers that were designed based on the conserved motifs in the NBS domain of resistance (R) proteins were used to isolate NBS-type sequences from IL5211S. A total of 28 sequences were identified and named as cucumber (C. sativus= CS) resistance gene analogs as CSRGAs. Polygenetic analyses separated these sequences into four different classes. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that these CSRGAs expressed at different levels in leaves, roots, and stems. In addition, introgression fromC. hystrixinduced expression of the partial CSRGAs in cultivated cucumber, especially CSRGA23, increased four-fold when compared to the backcross parent CC3. Furthermore, the expression of CSRGA23 underP. cubensisinfection and abiotic stresses was also analyzed at different time points. Results showed that theP. cubensistreatment and four tested abiotic stimuli, MeJA, SA, ABA, and H2O2,triggered a significant induction of CSRGA23 within 72 h of inoculation. The results indicate that CSRGA23 may play a critical role in protecting cucumber againstP. cubensisthrough a signaling the pathway triggered by these molecules.ConclusionsFour classes of NBS-type RGAs were successfully isolated from IL5211S, and the possible involvement of CSRGA23 in the active defense response toP. cubensiswas demonstrated. These results will contribute to develop analog-based markers related to downy mildew resistance gene and elucidate the molecular mechanisms causing resistance in IL5211S in the future.
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DOI：
10.1073/pnas.93.21.11746
发表时间：
1996-10-15
影响因子：
11.1
作者：
Kanazin, V;Marek, LF;Shoemaker, RC
通讯作者：
Shoemaker, RC
影响因子：
3.1
作者：
Martínez Zamora, MG;Castagnaro, AP;Díaz Ricci, JC
通讯作者：
Díaz Ricci, JC
影响因子：
30.8
作者：
Leister, D;Ballvora, A;Gebhardt, C
通讯作者：
Gebhardt, C
影响因子：
56.9
作者：
GRANT, MR;GODIARD, L;DANGL, JL
通讯作者：
DANGL, JL
影响因子：
7.2
作者：
Meyers, BC;Dickerman, AW;Young, ND
通讯作者：
Young, ND