Anti-ganglioside antibodies in amyotrophic lateral sclerosis revisited.
Anti-ganglioside antibodies in amyotrophic lateral sclerosis revisited.
复制标题
DOI：
10.1371/journal.pone.0125339
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Petri S
中科院分区：
文献类型：
--
作者：
Kollewe K;Wurster U;Sinzenich T;Körner S;Dengler R;Mohammadi B;Petri S
肌萎缩侧索硬化症（Amyotrophic Lateral Sclerosis，ALS）是一种典型的5 - 6岁发病的神经退行性疾病。ALS的自身免疫起源的假说今天受到较少的关注，但免疫学现象似乎仍然涉及，保护性自身免疫等机制可能很重要。几位作者在ALS患者中反复描述了对各种神经节苷脂抗体的检测，但报告的频率和滴度差异很大。因此，我们在一大群临床特征良好的ALS患者中研究了六种常见抗体的存在情况，并将其与200名健康献血者进行了比较。应用GanglioCombiELISA法检测84例ALS患者血清中6种神经节苷脂的IgG和IgM抗体。结果表示为高度阳性对照的%比率，并分类为阴性（<30%）、临界（30-50%）、中度（50-100%）和强阳性（>100%）。从200名瑞士献血者获得的值作为参考组。在22例（26.2%）ALS患者中，可检测到抗神经节苷脂抗体升高：将所有亚特异性抗体一起，在9/84例（10.7%）患者中发现IgG抗体，在15/84例（17.9%）患者中发现IgM抗体。ALS患者的年龄、性别、发病部位或生存期与抗神经节苷脂阳性/阴性滴度之间无相关性。与健康献血者相比，抗神经节苷脂抗体的频率没有统计学显著差异，即使采用这种更全面的方法，我们的ALS队列中的抗神经节苷脂抗体频率和模式与健康对照组的测量值非常相似。与其他研究一致，我们没有观察到不同的ALS表型与抗神经节苷脂抗体升高或对生存率的影响有任何关联。
Amyotrophic Lateral Sclerosis (ALS) is a devastating neurodegenerative disorder with typical onset in the 5th- 6th decade of life. The hypothesis of an autoimmune origin of ALS receives less attention today, but immunological phenomena still seem to be involved and mechanisms such as protective autoimmunity may be important. Detection of antibodies against a variety of gangliosides has been repeatedly described in ALS-patients by several authors, but widely differing frequencies and titres have been reported. Therefore, we investigated the presence of six common antibodies with a commercially available test panel for GA1, GM1, GM2, GD1a, GD1b and GQ1b in a large group of clinically well-characterized ALS patients and compared them to a collective of 200 healthy blood donors. IgG and IgM antibodies to the six gangliosides asialoGM1 (GA1), GM1, GM2, GD1a, GD1b, GQ1b were determined by GanglioCombi ELISA in sera of 84 ALS patients. Results were expressed as a %-ratio of a highly positive control and categorized as negative (<30%), borderline (30–50%), moderately (50–100%) and strongly positive (>100%). The values obtained from 200 Swiss blood donors served as a reference group. In twenty-two (26.2%) ALS-patients elevated anti-ganglioside antibodies could be detected: Taking all subspecific antibodies together, IgG antibodies were found in 9/84 (10.7%) and IgM in 15/84 (17.9%) patients. There was no correlation between age, gender, site of onset or survival and anti-ganglioside-positive/-negative titres in ALS-patients. No statistically significant difference in the frequency of anti-ganglioside antibodies compared to the group of healthy blood donors was found. Even with this more comprehensive approach, anti-ganglioside antibody frequencies and patterns in our ALS cohort closely resembled the values measured in healthy controls. In accordance with other studies, we did not observe any association of a distinct ALS phenotype with elevated anti-ganglioside antibodies or an impact on survival.
登录
查看更多内容
影响因子：
4.4
作者：
Cedarbaum, JM;Stambler, N;Nakanishi, A
通讯作者：
Nakanishi, A
影响因子：
4.4
作者：
ILYAS, AA;MITHEN, FA;COOK, SD
通讯作者：
COOK, SD
影响因子：
2.2
作者：
Benkler, Michal;Agmon-Levin, Nancy;Shoenfeld, Yehuda
通讯作者：
Shoenfeld, Yehuda
影响因子：
3.3
作者：
Farbu, E;Rekand, T;Vedeler, CA
通讯作者：
Vedeler, CA
影响因子：
158.5
作者：
GURNEY, ME;BELTON, AC;ANTEL, JP
通讯作者：
ANTEL, JP