Transcriptome Analysis During Follicle Development in Turkey Hens With Low and High Egg Production.
Transcriptome Analysis During Follicle Development in Turkey Hens With Low and High Egg Production.
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DOI：
10.3389/fgene.2021.619196
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发表时间：
2021
影响因子：
3.7
通讯作者：
Porter TE
中科院分区：
文献类型：
--
作者：
Brady K;Liu HC;Hicks JA;Long JA;Porter TE
低产蛋量和高产蛋量的母鸡表现出与卵巢类固醇生成相关的基因表达差异。高产蛋母鸡分别在最大卵泡的颗粒层（F1 G）和小白色卵泡的颗粒层（F5 I）中显示出与孕酮和雌二醇产生相关的基因的表达增加，而低产蛋母鸡分别在第五大卵泡的颗粒层（F5 G）和内膜层（F5 I）中显示出与孕酮和雄激素产生相关的基因的表达增加。对来自低产蛋母鸡和高产蛋母鸡的F1 G、F5 G、F5 I和E1 G样品进行转录组分析，以鉴定卵巢类固醇生成的新型调节剂。总之，在所检查的四种细胞类型中，在低产蛋母鸡和高产蛋母鸡之间鉴定了12，221个差异表达基因（DEG）。通路分析暗示了下丘脑-垂体-甲状腺（HPT）轴的差异调节，特别是甲状腺激素转运蛋白和甲状腺激素受体，以及低和高产蛋母鸡的雌二醇信号。HPT轴在产蛋量高的母鸡中在不太成熟的卵泡中表达上调，而在产蛋量低的母鸡中在更成熟的卵泡中表达上调。雌激素信号仅在高产蛋母鸡中表现出上调。在体外用甲状腺激素处理来自低产蛋母鸡和高产蛋母鸡的卵巢上皮细胞使来自高产蛋母鸡的细胞中的雌二醇产生降低到来自低产蛋母鸡的细胞中所见的水平，而甲状腺激素处理不影响来自低产蛋母鸡的细胞中的雌二醇产生。参与类固醇生成的主要细胞类型的转录组分析推断HPT轴和雌二醇信号转导参与调节不同产蛋水平的母鸡中观察到的差异类固醇激素产生。
Low and high egg producing hens exhibit gene expression differences related to ovarian steroidogenesis. High egg producing hens display increased expression of genes involved in progesterone and estradiol production, in the granulosa layer of the largest follicle (F1G) and small white follicles (SWF), respectively, whereas low egg producing hens display increased expression of genes related to progesterone and androgen production in the granulosa (F5G) and theca interna layer (F5I) of the fifth largest follicle, respectively. Transcriptome analysis was performed on F1G, F5G, F5I, and SWF samples from low and high egg producing hens to identify novel regulators of ovarian steroidogenesis. In total, 12,221 differentially expressed genes (DEGs) were identified between low and high egg producing hens across the four cell types examined. Pathway analysis implied differential regulation of the hypothalamo-pituitary-thyroid (HPT) axis, particularly thyroid hormone transporters and thyroid hormone receptors, and of estradiol signaling in low and high egg producing hens. The HPT axis showed up-regulation in high egg producing hens in less mature follicles but up-regulation in low egg producing hens in more mature follicles. Estradiol signaling exclusively exhibited up-regulation in high egg producing hens. Treatment of SWF cells from low and high egg producing hens with thyroid hormone in vitro decreased estradiol production in cells from high egg producing hens to the levels seen in cells from low egg producing hens, whereas thyroid hormone treatment did not impact estradiol production in cells from low egg producing hens. Transcriptome analysis of the major cell types involved in steroidogenesis inferred the involvement of the HPT axis and estradiol signaling in the regulation of differential steroid hormone production seen among hens with different egg production levels.
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影响因子：
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作者：
Matulis, Christina K.;Mayo, Kelly E.
通讯作者：
Mayo, Kelly E.
DOI：
10.1016/j.beem.2015.04.001
发表时间：
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
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CALVO, FO