Towards early detection of cervical cancer: Fractal dimension of AFM images of human cervical epithelial cells at different stages of progression to cancer.
Towards early detection of cervical cancer: Fractal dimension of AFM images of human cervical epithelial cells at different stages of progression to cancer.
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DOI：
10.1016/j.nano.2015.04.012
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Sokolov I
中科院分区：
文献类型：
--
作者：
Guz NV;Dokukin ME;Woodworth CD;Cardin A;Sokolov I
我们使用AFM HarmoniX模式来分析个体人宫颈上皮细胞在向癌症发展的三个阶段的表面，正常，永生（癌前）和癌细胞。测试了来自6个正常株、6个癌株和6个永生化株（通过质粒DNA-HPV-16转染来自6个健康个体的细胞而衍生）的原代细胞。该细胞模型允许将细胞表型良好控制至单细胞水平，这在临床筛选试验（离体）中是不切实际的。在固定的干燥细胞上收集物理（非特异性）粘附的AFM图。我们发现，一个表面参数称为分形维数可以用来隔离正常的永生癌前病变和恶性细胞的敏感性和特异性超过99%。所报道的分析方法可以直接应用于在液体细胞学筛查试验中收集的细胞，并通过常规光学方法鉴定为异常，以增加灵敏度。
We used AFM HarmoniX modality to analyse the surface of individual human cervical epithelial cells at three stages of progression to cancer, normal, immortal (pre-malignant) and carcinoma cells. Primary cells from 6 normal strains, 6 cancer, and 6 immortalized lines (derived by plasmid DNA-HPV-16 transfection of cells from 6 healthy individuals) were tested. This cell model allowed for good control of the cell phenotype down to the single cell level, which is impractical to attain in clinical screening tests (ex-vivo). AFM maps of physical (nonspecific) adhesion are collected on fixed dried cells. We show that a surface parameter called fractal dimension can be used to segregate normal from both immortal pre-malignant and malignant cells with sensitivity and specificity of more than 99%. The reported method of analysis can be directly applied to cells collected in liquid cytology screening tests and identified as abnormal with regular optical methods to increase sensitivity.
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影响因子：
3.5
作者：
Berdyyeva, TK;Woodworth, CD;Sokolov, I
通讯作者：
Sokolov, I
影响因子：
1.3
作者：
Hoda, RS;Colello, C;Houser, PM
通讯作者：
Houser, PM
影响因子：
2.4
作者：
Kant, R
通讯作者：
Kant, R
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
8.6
作者：
Dokukin ME;Guz NV;Gaikwad RM;Woodworth CD;Sokolov I
通讯作者：
Sokolov I