Fabrication of silica-coated gold nanorods functionalized with DNA for enhanced surface plasmon resonance imaging biosensing applications.

Fabrication of silica-coated gold nanorods functionalized with DNA for enhanced surface plasmon resonance imaging biosensing applications.
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用DNA功能化的二氧化硅涂层金纳米棒的制造,以增强表面等离子体共振成像生物传感应用。

DOI:
10.1021/la902675s
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发表时间:
2009-10-06
期刊:
Langmuir : the ACS journal of surfaces and colloids
影响因子:
--
通讯作者:
Corn RM
Corn RM
中科院分区:
其他
文献类型:
--
作者:
Sendroiu IE;Warner ME;Corn RM

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提出了一种制备金纳米棒的新方法,该方法首先用薄二氧化硅膜涂覆,然后用单链DNA(ssDNA)功能化。用3-5 nm的二氧化硅涂覆纳米棒提高了它们的溶解度和稳定性。胺修饰的ssDNA通过与醛三甲氧基硅烷单层的还原胺化反应附着到二氧化硅涂覆的金纳米棒上。纳米棒在780 nm处表现出强烈的吸收带,并用于增强DNA微阵列上的表面等离子体共振成像(SPRI)测量的灵敏度。
A novel method for preparing gold nanorods that are first coated with a thin silica film and then functionalized with single stranded DNA (ssDNA) is presented. Coating the nanorods with 3-5 nm of silica improves their solubility and stability. Amine-modified ssDNA is attached to the silica-coated gold nanorods via a reductive amination reaction with an aldehyde trimethoxysilane monolayer. The nanorods exhibit an intense absorption band at 780 nm, and are used to enhance the sensitivity of surface plasmon resonance imaging (SPRI) measurements on DNA microarrays.
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