Characterization of active miniature inverted-repeat transposable elements in the peanut genome.
Characterization of active miniature inverted-repeat transposable elements in the peanut genome.
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DOI：
10.1007/s00122-012-1798-6
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发表时间：
2012-05
影响因子：
5.4
通讯作者：
Isobe, Sachiko
中科院分区：
文献类型：
--
作者：
Shirasawa, Kenta;Hirakawa, Hideki;Tabata, Satoshi;Hasegawa, Makoto;Kiyoshima, Hiroyuki;Suzuki, Sigeru;Sasamoto, Sigemi;Watanabe, Akiko;Fujishiro, Tsunakazu;Isobe, Sachiko
微型反向重复转座元件 (MITE)，其中一些被称为活性非自主 DNA 转座子，存在于植物和动物的基因组中。在花生 (Arachishypogaea) 中，AhMITE1 已在脂肪酸去饱和酶基因中被发现，并具有切除活性。然而，花生基因组的 AhMITE1 分布和切除频率尚未确定。为了表征 AhMITE1，研究了它们的基因组多样性和转座能力。 Southern印迹分析表明AhMITE1在A.hypogaea、A.magna和A.monticola的基因组中具有高拷贝数，但在A.duranensis中却没有。从 A.hypogaea 富含 MITE 的基因组文库中鉴定出总共 504 个 AhMITE1。代表性的 AhMITE1 平均长度为 205.5 bp，GC 含量为 30.1%，富含 AT、9 bp 靶位点重复和 25 bp 末端反向重复序列。使用针对每个 AhMITE1 的两个侧翼序列设计的引物对进行 PCR 分析。这些分析在 4 个花生品系的 411 个插入基因座中检测到了 169 个的多态性。在随后对 60 个经伽马射线照射的突变株系的分析中，四个 AhMITE1 切除显示在测试的 109 个基因座处存在足迹突变。本研究表征了花生中的 AhMITE1，并讨论了它们作为 DNA 标记和诱变剂在花生及其近缘种的遗传学、基因组学和育种中的用途。本文的在线版本 (doi:10.1007/s00122-012-1798-6) 包含补充材料，可供授权用户使用。
Miniature inverted-repeat transposable elements (MITEs), some of which are known as active non-autonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), AhMITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four AhMITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives. The online version of this article (doi:10.1007/s00122-012-1798-6) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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通讯作者：
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作者：
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作者：
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通讯作者：
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微卫星作为培养花生中的DNA标记（Arachis hypogaea L.）。
影响因子：
5.3
作者：
He, Guohao;Meng, Ronghua;Newman, Melanie;Gao, Guoqing;Pittman, Roy N;Prakash, C S
通讯作者：
Prakash, C S