Measurement of cerebral oxygen pressure in living mice by two-photon phosphorescence lifetime microscopy.
Measurement of cerebral oxygen pressure in living mice by two-photon phosphorescence lifetime microscopy.
复制标题
用双光子磷光寿命显微镜测量活体小鼠脑氧分压。
DOI：
10.1016/j.xpro.2022.101370
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发表时间：
2022-06-17
期刊：
影响因子：
--
通讯作者：
Weber, Bruno
中科院分区：
文献类型：
--
作者：
Erlebach, Eva;Ravotto, Luca;Wyss, Matthias T.;Condrau, Jacqueline;Troxler, Thomas;Vinogradov, Sergei A.;Weber, Bruno
量化氧分压（pO2）的能力对于健康和疾病代谢过程的研究至关重要。在这里，我们提出了一种麻醉和清醒小鼠大脑皮层组织和脉管系统中氧分布的成像方案。我们描述了氧的体内双光子磷光寿命显微镜（2PLM）使用探针Oxyphor 2P。这种微创方案在精度、分辨率和成像深度方面优于现有的方法。有关使用和执行本协议的完整详情，请参阅。Oxyphor 2P的双光子磷光成像允许在体内测量氧气氧气成像可以在麻醉或清醒状态下进行，行为小鼠静脉注射可以在血管中进行氧气成像大池注射可以在脑血管外和血管内进行氧气成像定量氧分压（pO2）的能力对于研究健康和疾病的代谢过程至关重要。在这里，我们提出了一种麻醉和清醒小鼠大脑皮层组织和脉管系统中氧分布的成像方案。我们描述了氧的体内双光子磷光寿命显微镜（2PLM）使用探针Oxyphor 2P。这种微创方案在精度、分辨率和成像深度方面优于现有的方法。
The ability to quantify partial pressure of oxygen (pO2) is of primary importance for studies of metabolic processes in health and disease. Here, we present a protocol for imaging of oxygen distributions in tissue and vasculature of the cerebral cortex of anesthetized and awake mice. We describe in vivo two-photon phosphorescence lifetime microscopy (2PLM) of oxygen using the probe Oxyphor 2P. This minimally invasive protocol outperforms existing approaches in terms of accuracy, resolution, and imaging depth. For complete details on the use and execution of this protocol, please refer to. Two-photon phosphorescence imaging of Oxyphor 2P allows for oxygen measurement in vivo Oxygen imaging can be performed in anesthetized or awake, behaving mice Intravenous injection enables oxygen imaging in the vasculature Cisterna magna injection enables extra- and intravascular oxygen imaging in the brain The ability to quantify partial pressure of oxygen (pO2) is of primary importance for studies of metabolic processes in health and disease. Here, we present a protocol for imaging of oxygen distributions in tissue and vasculature of the cerebral cortex of anesthetized and awake mice. We describe in vivo two-photon phosphorescence lifetime microscopy (2PLM) of oxygen using the probe Oxyphor 2P. This minimally invasive protocol outperforms existing approaches in terms of accuracy, resolution, and imaging depth.
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影响因子：
14.8
作者：
Holtmaat, Anthony;Bonhoeffer, Tobias;Chow, David K.;Chuckowree, Jyoti;De Paola, Vincenzo;Hofer, Sonja B.;Huebener, Mark;Keck, Tara;Knott, Graham;Lee, Wei-Chung A.;Mostany, Ricardo;Mrsic-Flogel, Tom D.;Nedivi, Elly;Portera-Cailliau, Carlos;Svoboda, Karel;Trachtenberg, Joshua T.;Wilbrecht, Linda
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Wilbrecht, Linda
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通讯作者：
Sakadzic, Sava
影响因子：
17.1
作者：
Iliff JJ;Wang M;Liao Y;Plogg BA;Peng W;Gundersen GA;Benveniste H;Vates GE;Deane R;Goldman SA;Nagelhus EA;Nedergaard M
通讯作者：
Nedergaard M
影响因子：
3.4
作者：
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通讯作者：
Weber, Bruno