Proteomic Responses Under Cold Stress Reveal Unique Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus vannamei).
Proteomic Responses Under Cold Stress Reveal Unique Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus vannamei).
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冷应激下的蛋白质组反应揭示了太平洋白虾（Litopenaeus vannamei）独特的耐冷机制
DOI：
10.3389/fphys.2018.01399
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发表时间：
2018
影响因子：
4
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Peng JX;He PP;Wei PY;Zhang B;Zhao YZ;Li QY;Chen XL;Peng M;Zeng DG;Yang CL;Chen X
南美白色对虾（Litopenaeus vannamei）是世界上养殖面积最大的对虾品种之一，其低温胁迫是其生存的主要原因。为了解太平洋白色对虾耐冷性的分子机制，对耐冷型太平洋白对虾贵海2号（GH 2）和冷敏感型太平洋白对虾贵海1号（GH 1）在常温（28°C）、冷胁迫（16°C）和恢复至28°C条件下的蛋白质组进行了分析。总共鉴定了3，349种蛋白质，其中2，736种蛋白质被定量。基于基因本体论的注释，差异表达蛋白主要属于生物过程、细胞组分和分子功能。KEGG通路注释表明，主要变化在溶酶体、核糖体和氧化磷酸化中观察到。亚细胞定位分析表明，细胞质，细胞外区域和线粒体中存在的蛋白质显着增加。结合基于富集的聚类分析和qRT-PCR分析，我们发现谷胱甘肽S-转移酶、锌蛋白酶、m7 GpppX二磷酸酶、AP 2转录复合物和锌指转录因子在太平洋白色对虾的冷应激反应中起主要作用。此外，结构蛋白，包括不同类型的凝集素和DAPPUDRAFT，是必不可少的太平洋白色对虾的耐冷胁迫。研究结果揭示了太平洋白色对虾对低温胁迫反应的分子机制，为培育耐冷性增强的新品种提供了新的思路。
The Pacific white shrimp (Litopenaeus vannamei), one of the most widely cultured shrimp species in the world, often suffers from cold stress. To understand the molecular mechanism of cold tolerance in Pacific white shrimp, we conducted a proteomic analysis on two contrasting shrimp cultivars, namely, cold-tolerant Guihai2 (GH2) and cold-sensitive Guihai1 (GH1), under normal temperature (28°C), under cold stress (16°C), and during recovery to 28°C. In total, 3,349 proteins were identified, among which 2,736 proteins were quantified. Based on gene ontology annotations, differentially expressed proteins largely belonged to biological processes, cellular components, and molecular functions. KEGG pathway annotations indicated that the main changes were observed in the lysosome, ribosomes, and oxidative phosphorylation. Subcellular localization analysis showed a significant increase in proteins present in cytosol, extracellular regions, and mitochondria. Combining enrichment-based clustering analysis and qRT-PCR analysis, we found that glutathione S-transferase, zinc proteinase, m7GpppX diphosphatase, AP2 transcription complex, and zinc-finger transcription factors played a major role in the cold stress response in Pacific white shrimp. Moreover, structure proteins, including different types of lectin and DAPPUDRAFT, were indispensable for cold stress tolerance of the Pacific white shrimp. Results indicate the molecular mechanisms of the Pacific white shrimp in response to cold stress and provide new insight into breeding new cultivars with increased cold tolerance.
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影响因子：
5.6
作者：
Bai, Bin;Wu, Jun;Deng, Qi-Yun
通讯作者：
Deng, Qi-Yun
影响因子：
2.9
作者：
Khraltsova, LS;Sablina, MA;Bovin, NV
通讯作者：
Bovin, NV
DOI：
10.1126/science.1197761
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colbourne JK;Pfrender ME;Gilbert D;Thomas WK;Tucker A;Oakley TH;Tokishita S;Aerts A;Arnold GJ;Basu MK;Bauer DJ;Cáceres CE;Carmel L;Casola C;Choi JH;Detter JC;Dong Q;Dusheyko S;Eads BD;Fröhlich T;Geiler-Samerotte KA;Gerlach D;Hatcher P;Jogdeo S;Krijgsveld J;Kriventseva EV;Kültz D;Laforsch C;Lindquist E;Lopez J;Manak JR;Muller J;Pangilinan J;Patwardhan RP;Pitluck S;Pritham EJ;Rechtsteiner A;Rho M;Rogozin IB;Sakarya O;Salamov A;Schaack S;Shapiro H;Shiga Y;Skalitzky C;Smith Z;Souvorov A;Sung W;Tang Z;Tsuchiya D;Tu H;Vos H;Wang M;Wolf YI;Yamagata H;Yamada T;Ye Y;Shaw JR;Andrews J;Crease TJ;Tang H;Lucas SM;Robertson HM;Bork P;Koonin EV;Zdobnov EM;Grigoriev IV;Lynch M;Boore JL
通讯作者：
Boore JL
影响因子：
3.5
作者：
Fujikake, N;Nagai, Y;Toda, T
通讯作者：
Toda, T
DOI：
10.1073/pnas.0802432105
发表时间：
2008-09-02
影响因子：
11.1
作者：
Chen, Zuozhou;Cheng, C. -H. Christina;Chen, Liangbiao
通讯作者：
Chen, Liangbiao