Functional clustering of dendritic activity during decision-making
Functional clustering of dendritic activity during decision-making
复制标题
决策过程中树突活动的功能聚类
作者：
Aaron Kerlin;Mohar Boaz;Daniel Flickinger;Bryan J MacLennan;Matthew B Dean;Courtney Davis;N. Spruston;Karel Svoboda
树突的活性特性支持局部非线性操作，但先前的成像和电生理测量产生了关于体内局部非线性患病率的相互矛盾的观点。我们在执行触觉决策任务的小鼠的运动皮层中的锥体细胞树突中成像钙信号。一个定制的显微镜使我们能够在15 Hz下对索马和高达300 μm的连续树突成像，同时分辨单个棘。新的分析方法被用来估计频率和空间尺度的活动在树突状分支和棘。大多数树突状细胞钙瞬变与全球事件一致。然而，树突和棘中与任务相关的钙信号被树突分支所分隔，并聚集在约10 μm的分支内。不同的行为相关的信号混合在一起，分布在整个树突状乔木，可能支持一个大的计算库和学习能力，在个别神经元。
The active properties of dendrites support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence of local nonlinearities in vivo. We imaged calcium signals in pyramidal cell dendrites in the motor cortex of mice performing a tactile decision task. A custom microscope allowed us to image the soma and up to 300 μm of contiguous dendrite at 15 Hz, while resolving individual spines. New analysis methods were used to estimate the frequency and spatial scales of activity in dendritic branches and spines. The majority of dendritic calcium transients were coincident with global events. However, task-associated calcium signals in dendrites and spines were compartmentalized by dendritic branching and clustered within branches over approximately 10 μm. Diverse behavior-related signals were intermingled and distributed throughout the dendritic arbor, potentially supporting a large computational repertoire and learning capacity in individual neurons.
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影响因子：
2.5
作者：
Vogelstein, Joshua T.;Packer, Adam M.;Paninski, Liam
通讯作者：
Paninski, Liam
影响因子：
48
作者：
Kazemipour, Abbas;Novak, Ondrej;Podgorski, Kaspar
通讯作者：
Podgorski, Kaspar
影响因子：
2.4
作者：
Botcherby, E. J.;Juskaitis, R.;Wilson, T.
通讯作者：
Wilson, T.
影响因子：
64.8
作者：
Jia, Hongbo;Rochefort, Nathalie L.;Konnerth, Arthur
通讯作者：
Konnerth, Arthur
DOI：
10.1073/pnas.1312599110
发表时间：
2013-08-13
影响因子：
11.1
作者：
Hill, Daniel N.;Varga, Zsuzsanna;Konnerth, Arthur
通讯作者：
Konnerth, Arthur