Expression of TGFbeta family in the developing internal ear of rat embryos.
Expression of TGFbeta family in the developing internal ear of rat embryos.
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DOI：
10.3346/jkms.2006.21.1.136
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发表时间：
2006-02
影响因子：
4.5
通讯作者：
Kim H
中科院分区：
文献类型：
--
作者：
Kim HJ;Kang KY;Baek JG;Jo HC;Kim H
为探讨转化生长因子β（TGF β）亚型在内耳的表达模式，应用免疫组织化学方法对大鼠胚胎内耳进行了研究。分别于受孕后第13、15、17、19天取大鼠胚胎，取内耳进行TGFβ1、β2、β3免疫组化染色。结果显示，13日龄胚胎对TGFβ1呈非常弱的阳性。妊娠15天后，对TGFβ1无反应性。从妊娠第15天开始，在整个妊娠期间观察到对TGFβ2的免疫反应性。半规管壶腹和蜗管呈明显的免疫组化反应。妊娠第15天，TGFβ3反应较强。然而，此后未观察到阳性反应。在大鼠胚胎内耳发育过程中，耳蜗管的顶端细胞壁、上皮细胞表面、基底膜及半规管等处均有较强的免疫反应性。
In order to investigate the expression patterns of the transforming growth factor (TGF)β isoforms in the internal ear, an immunohistochemical study of rat embryos was performed. Rat embryos were taken on the 13th, 15th, 17th, and 19th day after conception and their internal ears were immunohistochemically stained against TGFβ1, β2, and β3. As a result, the 13-day-old embryo showed a very weak positivity to TGFβ1. After the 15th day of pregnancy, no reactivity to TGFβ1 was defected. Immunoreactivity to TGFβ2 was observed from the 15th day of pregnancy throughout the rest of the period. The ampulla of the semicircular canal and the cochlear duct showed a notably strong immunohistochemical reaction. A strong reaction to TGFβ3 was observed on the 15th day of pregnancy. However, no positive reactions were observed thereafter. A strong immunoreactivity was observed especially on the apical cytoplasms, the surfaces of the epithelial cells, and basement membranes of the cochlear duct, as well as the semicircular canals of the developing internal ear of rat embryo.
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影响因子：
5
作者：
McCartney-Francis, N L;Frazier-Jessen, M;Wahl, S M
通讯作者：
Wahl, S M
影响因子：
--
作者：
Nilsen-Hamilton, M.
通讯作者：
Nilsen-Hamilton, M.
DOI：
10.1002/(sici)1096-9926(200004)61:4
发表时间：
2000-04-01
期刊：
TERATOLOGY
影响因子：
--
作者：
Frenz, DA;Liu, W
通讯作者：
Liu, W
影响因子：
2.7
作者：
Kil, SH;Collazo, A
通讯作者：
Collazo, A
影响因子：
1.4
作者：
FRENZ, DA;VANDEWATER, TR;GALINOVICSCHWARTZ, V
通讯作者：
GALINOVICSCHWARTZ, V