Vasoactive intestinal polypeptide entrains circadian rhythms in astrocytes.
Vasoactive intestinal polypeptide entrains circadian rhythms in astrocytes.
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DOI：
10.1177/0748730409332042
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发表时间：
2009-04
影响因子：
3.5
通讯作者：
Herzog ED
中科院分区：
文献类型：
--
作者：
Marpegan L;Krall TJ;Herzog ED
许多哺乳动物细胞类型在昼夜节律起搏器驱动下表现出每日的基因表达节律。例如，培养的星形胶质细胞在Period1和Period2表达中显示昼夜节律。然而，尚不清楚星形胶质细胞中细胞间因子如何或哪些同步并维持节律性。由于星形胶质细胞对血管活性肠肽（VIP）高度敏感，血管活性肠肽是一种由神经元释放的神经肽，对于协调日常循环很重要，因此我们假设 VIP 会影响星形胶质细胞的昼夜节律。我们使用源自含有 PERIOD2 (PER2) 蛋白生物发光报告基因的敲入小鼠的星形胶质细胞培养物，以评估 VIP 对星形胶质细胞节律特性的影响。 VIP 诱导 PER2 表达整体节律的峰谷幅度呈剂量依赖性增加，最大效应接近 100nM VIP，阈值在 0.1 至 1 nM 之间。 VIP 还诱导 PER2 节律的剂量和相位依赖性变化，并且每日给予 VIP 可使星形胶质细胞的生物发光节律达到预测的相位角。这是神经肽可以将神经胶质细胞引导至相位响应曲线预测的相位的首次证明。我们得出的结论是，VIP 在体外有效地夹带星形胶质细胞，并且是协调大脑神经胶质细胞日常节律的候选者。
Many mammalian cell types show daily rhythms in gene expression driven by a circadian pacemaker. For example, cultured astrocytes display circadian rhythms in Period1 and Period2 expression. It is not known, however, how or which intercellular factors synchronize and sustain rhythmicity in astrocytes. Because astrocytes are highly sensitive to vasoactive intestinal polypeptide (VIP), a neuropeptide released by neurons and important for the coordination of daily cycling, we hypothesized that VIP entrains circadian rhythms in astrocytes. We used astrocyte cultures derived from knock-in mice containing a bioluminescent reporter of PERIOD2 (PER2) protein, to assess the effects of VIP on the rhythmic properties of astrocytes. VIP induced a dose-dependent increase in the peak-to-trough amplitude of the ensemble rhythms of PER2 expression with maximal effects near 100nM VIP and threshold values between 0.1 and 1 nM. VIP also induced dose- and phase-dependent shifts in PER2 rhythms and daily VIP administration entrained bioluminescence rhythms of astrocytes to a predicted phase angle. This is the first demonstration that a neuropeptide can entrain glial cells to a phase predicted by a phase response curve. We conclude that VIP potently entrains astrocytes in vitro and is a candidate for coordinating daily rhythms among glia in the brain.
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影响因子：
4.8
作者：
Gerhold, LM;Wise, PM
通讯作者：
Wise, PM
影响因子：
3.4
作者：
Granados-Fuentes, D;Saxena, MT;Herzog, ED
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Herzog, ED
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Abraham, U;Prior, JL;Herzog, ED
通讯作者：
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作者：
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通讯作者：
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64.5
作者：
Balsalobre, A;Damiola, F;Schibler, U
通讯作者：
Schibler, U