Analytic Microdensitometer for Quantitative Cytochemistry

将获得一种分析性显微密度计，以定量测量在生物组织的显微切片中可见的各种反应产物。这台机器将应用于一系列项目：1)暴露于四氢大麻酚后卵巢细胞酶水平的变化；2)糖尿病患者周细胞的退化过程；3)猴子中枢神经系统中递质相关酶的变化；4)猴子行为变化与病变诱导的中枢神经系统递质相关酶变化的关系；5)由于接触睾酮或雌二醇而引起的颈上神经节的改变；以及6)由于年龄或损伤而引起的运动单位的改变。生物研究仪器中令人兴奋的发展之一是将显微镜与计算机连接起来，以便轻松地对显微图像进行定量分析。所有项目都严重依赖密度计作为研究工具，并将利用机器的能力以极高的精确度和重复性测量不同波长的截面产品。这一仪器的使用将使几位优秀的研究人员大大加强他们的研究计划。

An analytical microdensitometer will be acquired to quantitatively measure a variety of reaction products visualized in microscopic sections of biological tissues. The machine is to be applied to a number of projects: 1) Changes in enzyme levels in ovarian cells as a result of exposure to tetrahydrocannabinol: 2) The process of degeneration in pericytes of diabetics; 3) Transmitter related enzymes in the central nervous system of monkeys; 4) The relationship of behavioral change to lesion-induced alterations in transmitter related enzymes in the central nervous system of monkeys; 5) Changes in the superior cervical ganglion as a result of exposure to testosterone or estradiol; and 6) Changes in the motor unit as a result of age or lesion. One of the exciting developments in instrumentation for biological research is the ability to couple microscopes to computers to allow easy quantitative analysis of microscopic images. All projects are critically dependent upon the densitometer as an investigative tool and will exploit the machine's ability to measure product on sections at various wavelengths with a great degree of accuracy and reproducibility. The availability of this instrument will allow several excellent investigators to greatly strengthen their research programs.