Analysis and Parameter Estimation of Immobilized Enzyme Reactions

新发展的生物技术领域涉及到利用微生物生产新产品的尝试。关键是开发大型生物反应器的新配置，这种反应器比过去的反应器更有效地利用微生物，过去的反应器必须分批运行，由非常稀薄的水系统组成(因此必须使用非常昂贵的分离部分)。将酶固定在固体支撑结构上允许连续操作，目的是减轻上述第一个限制。利用这种固定化酶设计大型生物反应器还处于初级阶段。这项研究试图使用在多相化学催化中开发的方法学来处理此类生物反应器。用于这些实验研究的体系是固定化酶体系，其行为是准一级反应体系。固相，在这里是一种生物制品，(类似于化学体系中的催化剂)是反应发生的地方。将使用的特定系统包括固定化葡萄糖异构酶、固定化转化酶和固定化乳糖酶。

The newly-evolving area of biotechnology involves an attempt to use microorganisms to produce new products. Essential is the development of novel configurations of large bioreactors that utilize the microorganisms more efficiently than the reactors of the past which had to be run in batches and consisted of very dilute aqueous systems (and therefore had to be followed by very expensive separation sections). Immobilizing enzymes on a solid support structure permits continuous operation and is aimed at alleviating the first of the above limitations. The design of large scale bioreactors, utilizing such immobilized enzymes is in its infancy. This research is attempting to use methodology developed in heterogeneous chemical catalysis for such bioreactors. The system to be used for these experimental studies are immobilized enzyme systems which behave as pseudo-first-order reacting systems. The solid phase, in this care a bioparticle, (analogous to the catalyst in chemical systems) is where the reaction occurs. The specific systems that will be used include immobilized glucose isomerase, immobilized invertase, and immobilized lactase.