Dissertation Improvement Research (Thailand): Circulating Antigens During Trypanosoma lewisi Infections Characterized by Monoclonal Antibody Procedures

这项提案要求提供资金，以允许泰国曼谷玛希多尔大学热带医学院微生物学和免疫学系科学家Varee Prasertsiriroj女士在印第安纳州立大学生命科学系教授Donald G.Dusanic博士的指导下，从事与泰国发展有关的微生物学和免疫学的学位论文研究，为期12个月。本研究的目的是鉴定和鉴定在感染原虫路易斯锥虫的大鼠血浆中循环的重要寄生虫抗原。这些循环抗原可能具有免疫抑制或免疫保护作用。将尝试(1)使用单抗通过亲和层析来纯化这些抗原，(2)从物理和生物化学上确定它们，以及(3)确定它们在免疫中的作用。寄生性原虫是影响人和牲畜的传染病的主要原因。它们在限制许多国家，特别是热带地区的社会和经济发展方面发挥着重要作用。这项研究将为研究寄生虫感染的控制机制提供机会，因此将有助于泰国等热带国家的发展。杜萨尼克博士在这一提议领域是一位备受尊敬的科学家，他在美国和国外的寄生虫学领域培养出了数量可观的优秀毕业生。该项目与发展中国家科学计划的目标相关，该计划旨在通过交流科学信息、思想、技能和技术，以及通过在互利问题上的合作，提高美国科学家和工程师与发展中国家同行之间的合作水平。

This proposal requests funds to permit Ms. Varee Prasertsiriroj, Scientist (on leave of absence for study), Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, to pursue, for a period of 12 months, dissertation research in microbiology and immunology, relevant to development of Thailand, under the direction of Dr. Donald G. Dusanic, Professor, Department of Life Sciences, Indiana State University. The purpose of this study is to identify and characterize important parasite antigens circulating in the plasma of rats infected with Trypanosoma lewisi, a protozoan parasite. These circulating antigens may have a role in immunosuppression or immunoprotection. Attempts will be made to (1) purify these antigens by affinity chromatography using monoclonal antibodies., (2) define them physically and biochemically, and (3) determine their roles in immunity. Parasitic protozoa are a major cause of the infectious diseases which affect man and livestock. They play an important role in limiting the social and economic development of many countries, especially in tropical regions. The research will provide an opportunity to study mechanisms of control of parasitic infections and, therefore, will contribute to development of tropical countries such as Thailand. Dr. Dusanic is a highly respected scientist in the field of this proposal, and he has produced an impressive number of well placed graduates, in the United States and abroad, in the field of parasitology. This project is relevant to the objectives of the Science in Developing Countries Program which seeks to increase the level of cooperation between U.S. scientists and engineers and their counterparts in developing countries through the exchange of scientific information, ideas, skills, and techniques and through collaboration on problems of mutual benefit.