U.S.-Netherlands Cooperative Research: Cell Biology of Receptor Mediated Endocytosis

这是一个为期三年的细胞生物学合作研究项目 艾伦·L教授Schwartz，儿科教授， 华盛顿大学医学院药理学教授，圣路易斯。 路易斯，MO和教授H。Geuze和G. J. A. M. Strous，Center for 荷兰乌得勒支大学电子显微镜。 的 合作的科学家及其研究小组成员将 继续努力阐明受体的细胞生物学 介导的内吞作用（RME），并定义这一过程中， 生物化学和形态学术语。 调查人员将使用 肝去唾液酸糖蛋白受体作为研究RME的模型。 受体介导的内吞作用是细胞外 大分子和颗粒进入细胞内 环境 该过程负责细胞摄取， 包括必需细胞在内的大类物质的递送 营养素（例如铁、维生素B-12）、毒素（例如白喉毒素）、 细胞生长因子/激素（例如胰岛素），病毒（例如流感）， 和经历从循环中清除的蛋白质（例如， 去唾液酸糖蛋白）。 这项合作研究预计将导致一个 增加对细胞-细胞分子基础的理解 从外界传递生物化学信息 这样的基本生物学知识 RME将直接适用于阐明细胞的 正常细胞中的过程，并且另外提供关于其 to base基础new新approaches方法to directed定向drug药物therapy治疗. 这里的方法将 联合收割机结合生物化学和分子生物学方面（教授 Schwartz的专业知识）与超微结构免疫细胞化学（ 荷兰调查人员的专业知识）。 Geuze教授的实验室 乌得勒支配备了几台最先进的电子显微镜 和本研究中将使用的超低温切片机。 资金 这笔奖金将用于支付必要的交通和生活费用。 美国首席研究员及其研究成员的费用 组 荷兰政府通过荷兰大学 乌得勒支也为该项目提供支持。

This is a three-year cooperative research project in cellular biology between Professor Alan L. Schwartz, Professor of Pediatrics and Professor of Pharmacology, Washington University School of Medicine, St. Louis, MO, and Professors H. J. Geuze and G. J. A. M. Strous, Center for Electron Microscopy, University of Utrecht, The Netherlands. The cooperating scientists and members of their research groups will continue their efforts to elucidate the cell biology of receptor mediated endocytosis (RME) and to define this process in both biochemical and morphological terms. The investigators will use the hepatic asialoglycoprotein receptor as their model for the study of RME. Receptor mediated endocytosis is a process by which extracellular macromolecules and particles gain entry to the intracellular environment. The process is responsible for the cellular uptake and delivery of a broad class of substances which include essential cell nutrients (e.g. iron, Vitamin B-12), toxins (e.g. diphtheria toxin), cell growth factors/hormones (e.g. insulin), viruses (e.g. influenza), and proteins undergoing clearance from the circulation (e.g. asialoglycoprotein). This cooperative study is expected to lead to an increased understanding of the molecular basis of cell-cell communication and the transfer of biochemical information from outside of a cell to within it. Such an understanding of the basic biology of RME would have direct applicability to the elucidation of cellular processes in normal cells, and in addition provide a background on which to base new approaches to directed drug therapy. The approach here will combine a biochemical and molecular biological aspect (Professor Schwartz's expertise) with ultrastructural immunocytochemistry (the expertise of the Dutch investigators). Professor Geuze's laboratory in Utrecht is equipped with several state-of-the-art electron microscopes and ultra-cryo microtomes which will be utilized in this study. Funds from this award will support necessary transportation and subsistence expenses for the U.S. principal investigator and members of his research group. The government of The Netherlands, through the University of Utrecht, is also providing support for the project.