Phloem Physiology: Regulation of Sugar Transport and Allocation

韧皮部生理学：糖的运输和分配的调节：研究将集中于阐明叶源和韧皮部之间的连接是如何调节的，以控制光合作用碳水化合物在叶绿体和液泡池之间的分配和转移到韧皮部。我们将在分离的原生质体和液泡上使用膜片钳电极技术来研究这种机制。卢卡斯博士设计的一种特殊技术，将荧光探针结合到脂质体中，将被用于研究运输是通过胞间连丝还是通过质外体进行的。植物的生长和生产力取决于叶片中光合作用的碳水化合物从叶片通过韧皮部到茎、根、花或种子的运动，这些碳水化合物将在那里使用或储存。了解韧皮部是如何装卸的，这些过程是如何调节的，以及从汇到源的信号的性质，对于有计划地科学开发更高效的植物是基本的。

Phloem Physiology: Regulation of Sugar Transport and Allocation: Studies will focus on elucidating the manner in which the connection between the leaf source and phloem is regulated to control photosynthesized carbohydrate allocation between storage in chloroplast and vacuolar pools and translocation into the phloem. The mechanisms will be investigated using patch clamp electrode techniques on isolated protoplasts and vacuoles. A special technique devised by Dr. Lucas that uses fluorescent probes incorporated into lipsomesliposomes will be applied to study whether transport is symplastic via plasmodesmata or apoplastic. The growth and productivity of plants depends on the movement of carbohydrate photosynthesized in the leaf from the leaf via the phloem to the stem, root, flower or seed where it will be used or stored. Knowledge of how phloem is loaded and unloaded, how these processes are regulated, and the nature of the signal from sink to source is basic to the planned scientific development of more efficient plants.