Acquistion of Confocal Microscope for Research & Teaching

购买共焦显微镜用于研究

• 批准号: 0354790
• 负责人: William Lucas
• 金额: $ 29.6万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-15 至 2006-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0354790&HistoricalAwards=false
• 关键词: Acquistion; Confocal; Microscope; Research; &amp

This award provides partial support for the purchase of a confocal laser scanning microscope (CLSM) with spectral imaging capabilities, and an image analysis system to be housed in the Division of Biological Sciences-Microscopy and Imaging Facility at the University of California, Davis Campus. The instrument will enhance several research projects in basic and applied plant biology, including: (i) Elucidation of the molecular constituents that comprise the unique plasmodesmata-based non-cell-autonomous translocation pathway of plants, (ii) Characterization of the pathways involved in establishing cell fate in Arabidopsis organs, (iii) Identification of the Arabidopsis genes required for gametogenesis and embryogenesis, (iv) Molecular basis of ovule development and evolution, (v) Analysis of Aux/IAA degradation in plants, (vi) Regulation of compound leaf development. (vii) Phytochrome engineering in living cells, (viii) The role of molecular motors in the phragmoplast and during cotton fiber development, (ix) Identification of endogenous and viral component involved in plant systemic infection, (x) Analysis of signal transduction pathways underlying the operation of a tonoplast transport systems involved in plant response to salt stress, and (xi) Kinase signaling in the XA21-mediated plant defense response.

该奖项为购买具有光谱成像能力的共聚焦激光扫描显微镜（CLSM）和图像分析系统提供部分支持，该系统将被安置在加州大学戴维斯分校生物科学显微镜和成像设施部。该仪器将加强基础和应用植物生物学的几个研究项目，包括：（i）阐明包含植物独特的基于胞间连丝的非细胞自主易位途径的分子成分，（ii）表征参与在拟南芥器官中建立细胞命运的途径，（iii）鉴定配子发生和胚胎发生所需的拟南芥基因，（iv）胚珠发育和进化的分子基础，（v）植物中Aux/IAA降解的分析，（vi）复叶发育的调控。(vii)活细胞中的光敏色素工程，（viii）成膜体中和棉纤维发育期间分子马达的作用，（ix）参与植物系统性感染的内源和病毒组分的鉴定，（x）参与植物对盐胁迫的响应的液泡膜运输系统的操作的信号转导途径的分析，和（xi）XA 21介导的植物防御反应中的激酶信号传导。