An Investigation of the Cellular Events that Initiate and Control the Deposition of Patterned Walls in Plant Cells

本研究将利用一个独特的发育系统（百日草> > >elegans>的叶肉细胞在悬浮培养中分化形成具有明显图案次生壁的气管元件），以获得更完整的观点，了解高等植物细胞如何启动和控制图案壁的沉积。为了实现这一目标，将开展五个主要领域的研究：(1)光镜和电子显微镜来阐明分化的时间和细胞学；(2)微管作用的研究；(3)微丝作用的研究；(4)研究高尔基体在质膜中的作用和玫瑰花（与纤维素合成有关的颗粒）的功能寿命；(5)钙的研究。对分化的开始和继续至关重要的介导事件。光学和电子显微镜（结合免疫化学和细胞化学探针）将用于监测分化的变化。拮抗药物应用前后的相关细胞过程。这项研究的结果将增加我们对不同细胞特征如何共同控制一个明确定义的发育事件的认识。它们也将增强我们对植物形态发生控制的理解，这部分是由细胞壁微原纤维的模式决定的。

This research will exploit a unique developmental system (in which mesophyll cells of >Zinnia> >elegans> differentiate in suspension culture to form tracheary elements with distinctly patterned secondary walls) in order to obtain a more integrated view of how higher plant cells initiate and control the deposition of a patterned wall. Five major areas of reserach will be conducted to meet this goal: (1) Light and electron microscopy to clarify the timing and cytology of differentiation; (2) Investigation of the role of microtubules; (3) Investigation of the role of microfilaments; (4) Investigation of the role of the Golgi apparatus and the functional life of rosettes (particles associated with cellulose synthesis) in the plasma membrane; and (5) Investigation of calcium.mediated events critical to the initiation and continuation of differentiation. Light and electron microscopy (coupled with immunochemical and cytochemical probes) will be used to monitor changes in differentiation.related cellular processes before and after application of antagonistic drugs. The results of this study will increase our knowledge of how diverse cellular features function together in the control of a well.defined developmental event. They will also enhance our understanding of the control of plant morphogenesis, which is partly determined by the pattern of microfibrils in cell walls.