Study of Restriction Endonucleases

目标和相关性：该行动支持合作研究 理查德·J·罗伯茨博士来自长岛的冷泉港实验室 Island，NY和高级分子生物学中心的Sheikh Riazuddin博士 巴基斯坦拉合尔旁遮普大学生物学。研究 将涉及生产各种新的限制酶 用于基因工程的新特性。注意力将 这些方法允许发现具有识别能力的酶， 长度超过6个核苷酸的序列，因为这种酶， DNA很少对绘制大型基因组具有重要价值， 人类基因组 美国--巴基斯坦计划支持合作研究 这两个国家的科学家 相辅相成。 这个项目很好地满足了这个标准。 优点：这两位科学家是世界上最受尊敬的科学家之一。 分子生物学在各自的国家。 他们有 过去曾合作进行科学研究并发表成果 在国际期刊上发表联合文章。 该项目可能会 产生可持续的结果，这将有利于其他工作在 领域 供资：该项目由两项赠款供资; 旁遮普大学，INT-8713479号赠款： 1987财政年度：22，925港元（巴基斯坦货币） 冷泉港实验室，授予INT-8713519： FY 87：美元20，925加证监会$5，000 FY 88：美元22,181

Objective and Relevancy: This action supports collaborative research between Dr. Richard J. Roberts of Cold Spring Harbor Laboratory in Long Island, NY and Dr. Sheikh Riazuddin of the Center of Advanced Molecular Biology at the University of Punjab, Lahore, Pakistan. The research will involve production of a wide variety of new restriction enzymes with new specificities for use in genetic engineering. Attention will be paid to methods that allow the discovery of enzymes with recognition sequences longer than 6 nucleotides, since such enzymes which cleave DNA infrequently are of great value for mapping large genomes such as human genomes. The U.S.-Pakistan program supports cooperative research involving scientists from the two countries where capabilities complement each other. This project meets that criterion well. Merit: The two scientists are among the most highly regarded in the field of molecular biology in their respective countries. They have collaborated on scientific research in the past with results published as joint articles in international journals. This project is likely to produce publishable results which would benefit others working in the field. Funding: The project is being funded under two grants; University of Punjab, grant INT-8713479: FY87: SFC$22,925 (Pakistani Currency) Cold Spring Harbor Laboratory, grant INT-8713519: FY87: U.S.$20,925 plus SFC$5,000 FY88: U.S.$22,181