Facilities Center for NMR of Biological Solids.

单交叉极化和双交叉极化的组合， 魔角旋转产生了高分辨率的13 C和15 N 各种生物固体的核磁共振谱。 最近 这些实验的开发版本将用于识别 昆虫外骨骼和植物细胞壁的交联， 建立药物对细菌细胞壁的作用机制 代谢，以确定固定化酶中的结合位点， 测量动态和构象的灵活性， 酶的活性位点，以测量多种酶的流动性， 凝胶状态下脂质链中的位点，无特异性标记 的脂质，并监测药物的catastrophism， 除草剂和杀虫剂在哺乳动物组织中的非破坏性。 这些新应用中的许多将涉及NMR技术， 都是在这个实验室里研发出来的 这种能力 实验室将通过建立一个新的双交叉扩大 极化~ 1H-13 C-15 N固体谱仪 专门针对计划的需求的特点 新的应用。 这个光谱仪，沿着三个现有的 固体光谱仪，将包括一个新的NSF生物 设施生物固体核磁共振分析中心。 的 该中心所代表的综合专业知识是独一无二的， 多学科的。 此前，首席研究员 良好的合作努力，这可能会继续下去;和他的 实验室处于发展的最前沿， 尖端技术 他坚定地致力于扩大 在生物界的应用。 潜在 应用范围从仪器开发到化学 物理学、生物学/医学和生物技术。

The combination of single- and double-cross polarization with magic-angle spinning has resulted in high-resolution 13C and 15N NMR spectra of a variety of biological solids. Recently developed versions of these experiments will be used to identify cross-links in insect exoskeleton and plant cell walls, to establish the mechanism of drug action on bacterial cell-wall metabolism, to identify binding sites in immobilized enzymes, to measure the dynamics and conformational flexibility within the active sites of enzymes, to measure the mobility of multiple sites in lipid chains in the gel state without specific labeling of the lipids, and to monitor the catabolism of drugs, herbicides, and pesticides in mammalian tissue nondestructively. Many of these new applications will involve NMR techniques that have been developed in this laboratory. The capabilities of this laboratory will be expanded by building a new double-cross polarization 1H-13C-l5N solids spectrometer with operating characteristics specifically tailored to the needs of the planned new applications. This spectrometer, along with three existing solids spectrometers, will comprise a new NSF Biological Facilities Center for the NMR analysis of biological solids. The combined expertise represented in this Center is unique and multidisciplinary. Previously the Principal Investigator has had good collaborative efforts, which are likely to continue; and his laboratory is at the forefront of the develoment of this sophisticated technology. He is firmly committed to wider applications among the biological community. Potential applications range from instrument development, to chemical physics, biology/medicine, and to biotechnology.