Centers for Prokaryotic Genome Analysis and Molecular Phylogeny

本提案要求资金支持两个不同的，但高度相互作用的生物设施中心：原核基因组分析中心（CPGA）和分子系统发育中心（CMP）。CPGA的建立是为了促进对选定的古细菌和真细菌光合生物基因组确定的DNA序列的获取和分析。该中心将是跨学科的，其科学家来自分子生物学、微生物生理学、计算机科学、信息理论和工程学。该中心的活动将对原核生物进化、分子生物学和生理学的理解产生重大影响。该中心还将大力参与开发计算机。基于系统的序列数据处理。CMP是一个跨学科的小组，其成员在进化生物学方面有共同的兴趣，并使用生物化学和分子生物学方法来研究不同生物的系统发育。除核酸测序外，核心成员在其他生化方法方面具有全国公认的专业知识，特别是电泳和免疫学。这两个小组有两个共同感兴趣的领域：进化和序列分析的机制和方法。这些设施的重点将是基因组作图，基因测序，确定系统发育，并检查核糖体RNA基因的序列进化。所要求的仪器将使伊利诺伊大学成为一个卓越的分子进化中心。

This proposal requests funds to support two distinct, yet highly interacting, biological facility centers: the Center for Prokaryotic Genome Analysis (CPGA) and the Center for Molecular Phylogeny (CMP). The CPGA is being created to facilitate the acquisition and analysis of the DNA sequences determined for the genomes of selected archaebacteria as well as an eubacterial photosynthetic organism. The Center will be interdisciplinary, its scientists being drawn from molecular biology, microbial physiology, computer science, information theory, and engineering. The Center's activities will have a major impact on the understanding of prokaryotic evolution, molecular biology, and physiology. The Center will also be heavily involved in developing computer.based systems for the handling of sequence data. The CMP is an interdisciplinary group whose members share interests in evolutionary biology and use biochemical and molecular biological methods to examine the phylogenies of diverse sets of organisms. In addition to nucleic aid sequencing, core members have nationally recognized epertise in other biochemical methods, especially electrophoresis and immunology. The two groups have two areas of common interest..evolution and the mechanics and methodology of sequence analysis. The focus of these facilities will be on genome mapping, gene sequencing, and the determination of phylogenies, and examination of the sequence evolution of ribosomal RNA genes. The instrumentation requested will enable the University of Illinois to become a center for excellence in molecular evolution.