The Mechanism and Regulation of Ovulation in Fish

过去的研究涉及二十烷基类化合物、磷脂酰肌醇/蛋白激酶C系统和蛋白水解酶在鱼类排卵中的作用。这项提案研究将增加我们对这三种介质在鱼类排卵中的作用的了解。具体地说，将使用放射性标记前体掺入法研究鲑鱼和金鱼卵巢脂肪氧合代谢产物的产生，然后用高效液相色谱分离代谢物。用放射免疫分析法研究类固醇诱导的黄花鱼排卵过程中初级前列腺素的产生。一个涉及放射性肌醇标记卵泡磷脂酰肌醇的掺入系统将被研究，以便未来的研究可以使用该系统来研究激动剂刺激后卵泡肌醇磷酸盐的循环。利用底物聚丙烯酰胺凝胶电泳法对河豚和金鱼的卵泡总蛋白降解活性进行跟踪研究。利用一种特定的底物分析方法，通过排卵来跟踪河豚滤泡中的胶原蛋白分解活性，以确定这种蛋白水解酶在卵泡释放卵子中的作用。目的是更好地了解鱼类排卵的内分泌过程。

Past research implicates eicosanoids, the phosphatidylinositol/protein kinase C system and proteolytic enzymes in fish ovulation. The proposal research will increase our understanding of the role of these three mediators in fish ovulation. Specifically, the production of lipoxygense metabolites by brook trout and goldfish ovaries will be investigated using radio labeled precursor incorporation, followed by metabolite separation on high performance liquid chromatography. The production of primary prostaglandins during steroid-induced ovulation in yellow perch will be investigated using radioimmunoassay. An incorporation system, involving the labeling of follicular phosphatidylinositol by radioactive myoinositol, will be investigated so that future research can use this system to study the cycling of follicular inositol phosphates following agonist stimulation. Total follicular proteolytic activity thoughout maturation and ovulation will be followed in brook trout and goldfish using substrate polyacrylamide gel electrophoresis. Collagenolytic activity will be followed through ovulation in brook trout follicles using a specific substrate assay to determine the role of this proteases in the release of the eggs from the follicles. The goal is a better understanding of the endocrine processes regulating ovulation in fish.