Structure, Regulation and Mechanism of the Action of Oxidizing Enzyme

细胞色素亚基的一级序列 黄细胞色素对甲酚甲基羟化酶已被 前52个氨基酸的序列也是如此 黄素蛋白亚基的结构。 X射线晶体学数据. [3]这些数据已经得到了解析， 三级结构已被解开。 其余的 DNA测序正在进行中。 的 黄细胞色素可逆分解为亚单位 已被广泛研究，其动力学 机制，使用闪光光解，停流，和稳定- 陈述动力学方法。 催化的反应序列 该酶已显示包括乙烯基衍生物。 的 MPTP神经毒性的生化事件， 非法哌替啶类似物中的污染物， 测定的和与酶学相关的体内毒性 数据 一种新的代谢物载体泵MPP+， MPTP的氧化产物进入线粒体基质， 已被发现并部分鉴定。 广泛 水杨酸盐的动力学研究已经完成 羟化酶，使用一种新的程序来分离酶。 “内”和“外”的动力学和分子特征 已经比较了“外部”肉毒碱酰基转移酶， 过氧化物酶体显示是外部的可能来源 酵素

The primary sequence of the cytochrome subunit of the flavocytochrome p-cresol methylhydroxylase has been determined, as has the sequence of the first 52 amino acids of the flavoprotein subunit. X-ray crystallographic data at 3 A resolution have been fitted to these data and much of the tertiary structure has been unravelled. The rest of the sequence is being determined by DNA sequencing. The reversible dissociation of the flavocytochrome into subunits has been extensively investigated, as has its kinetic mechanism, using flash photolysis, stopped-flow, and steady- state kinetic methods. The reaction sequence catalyzed by the enzyme has been shown to include a vinyl derivative. The biochemical events underlying the neurotoxicity of MPTP, a contaminant in an illicit meperidine analog, has been determined and in vivo toxicity correlated with enzymological data. A new metabolite carrier which pumps MPP+, the oxidation product of MPTP, into the mitochondrial matrix, has been discovered and partially characterized. Extensive kinetic studies have been completed on salicylate hydroxylase, using a novel procedure to isolate the enzyme. The kinetic and molecular characteristics of an "inner" and an "outer" carnitine acyltransferase have been compared and peroxisomes shown to be the likely source of the the outer enzyme.