Probing the Structure of Ribosomal RNA

核糖体是参与蛋白质合成的关键大分子成分。它们由蛋白质和核糖体rna组成。本建议的目标是绘制发生在核糖体上的rRNA单链区域的相对位置，使用短的，互补的低聚物作为探针。相互作用将通过荧光各向异性、能量转移技术和光亲和标记来分析。选定位点与各种核糖体蛋白的距离将通过中子衍射测量。这些研究将为核糖体和核糖体亚基的详细结构提供有价值的信息，从而增强我们对蛋白质合成过程的理解。

Ribosomes are key macromolecular components involved in the synthesis of proteins. They are made up of proteins and ribosomal RNAs. The goal of this proposal is to map the relative positions of single-stranded regions of rRNA that occur on the ribosome, using short, complimentary oligomers as probes. Interactions will be analyzed by fluorescence anisotropy, energy transfer techniques and photoaffinity labeling. The distance of selected sites from various ribosomal proteins will be measured by neutron diffraction. These studies will provide valuable information on the detailed structure of the ribosome and ribosomal subunits, and thus enhance our understanding of the process of protein synthesis.