Gene Targeting Via Site Specific Recombination in Plant Cells

PI建议评估基因靶向的可能性， 植物体细胞 该实验首先涉及引入 一个突变的选择标记基因，并建立了一套稳定的 转化体 这些突变株系将被二次转化 在相同的选择标记基因中具有互补突变体。 在这个 将施加时间选择，以便鉴定转化的细胞， 这些基因之间存在着位点特异性重组， 互补突变体，从而重建功能性可选择的 标记基因 这一事件的分子细节将被检查。 这项技术可以允许基因替换或失活， 植物细胞和将顺序引入的性状靶向单个 加快植物品种改良。

The PI proposes to evaluate the possibility of gene targeting in somatic plant cells. The experiment involves first the introduction of a mutant selectable marker gene and the establishment of a set of stable transformants. These mutant lines will then be secondarily transformed with a complimentary mutant in the same selectable marker gene. At this time selection will be imposed so as to identify transformed cells in which there has been site specific recombination between these complimentary mutants thereby reconstructing a functional selectable marker gene. The molecular details of this event will be examined. This technology could allow for gene replacement or inactivation in plant cells and targeting of sequentially introduced traits to a single locus to expedite plant variety improvement.