Signalling Mechanisms and Mobility of the EGF Receptor

本项目旨在利用数字成像光学显微镜和一种新的受体定位可视化方法来表征受体或表皮生长因子(EGF)的迁移率。此外，EGF刺激引起的胞浆游离钙离子活性的变化将在单个细胞的水平上进行测量。EGF是一种多肽，可以结合到不同细胞类型的特定质膜上。它在细胞中触发一系列反应，最终导致DNA合成的刺激。除了刺激细胞增殖外，EGF还可诱导早期和晚期反应，这些反应可能在EGF结合所引发的信号转导中发挥作用，最终导致有丝分裂。

This project aims to characterize the mobility of the receptor or epidermal growth factor (EGF) using digital imaging optical microscopy and a novel methods of visualization of receptor location. Additionally, the changes in free cytosolic calcium ion activity induced by EGF stimulation will be measured at the level of the individual cell. EGF is a polypeptide which binds to a specific plasma membrane on various different cell types. It triggers a number of responses in the cell ultimately leading to a stimulation of DNA synthesis. In addition to the stimulation of proliferation, EGF induces early and late responses which may play a role in the transduction of the signal initiated by the binding of EGF and culminating with mitogenesis.