Assessing and Manipulating Single-Cell Productivity in Recombinant Cell Populations

本研究涉及三个平行和概念相关的研究，以探索重组微生物群体的异质性和单细胞生产力的基础。它还寻求对遗传和环境参数与这种异质性之间的关系进行定量理解。该研究涉及快速单细胞测量实验方法的最新发展，将有助于进一步扩展和更广泛地应用这些极其高效和信息丰富的测量。大肠杆菌和酵母细胞也将使用一套最近在生物技术工业中开发的实验系统进行研究。三名研究生将进行拟议的实验，并将与当地一家生物技术公司直接合作。

This research involves three parallel and conceptually related studies to explore the bases for heterogeneity and single-cell productivity in recombinant microbial populations. It also seeks to develop quantitative understandings of the relationships between genetic and environmental parameters and this heterogeneity. The research involves very recent developments in experimental methodology for rapid single-cell measurements and will contribute to further expansion and broader application of these extremely efficient and informative measurements. E. coli and yeast cells will also be studied using a set of experimental systems which have been developed recently in the biotechnology industry. Three graduate students will conduct the proposed experiments and will be in direct collaboration with a local biotechnology company.