Research Initiation: Investigation of the Mechanisms Responsible for Increased Antibody Production by Hybridomas Exposed to Stress

研究启动奖：对暴露在压力下的杂交瘤抗体产生增加的机制的调查摘要单抗是利用哺乳动物细胞生产的最大容量的产品之一。据报道，暴露在环境压力下的杂交瘤抗体产生增加。该项目的目标是确定抗体产量增加的机制，并确定生产率增加的反应条件。将监测能量代谢和DNA合成的同时变化，以确定细胞生长调节、能量产生和蛋白质表达之间的相互关系。为了使细胞生长与产品形成脱钩，需要了解这些关系。细胞过度生长是不可取的，因为昂贵的培养液被浪费在生产生物质上。降低细胞生长速度也是可取的，因为许多原代、杂交和基因改变的细胞系在重复细胞分裂后降低了它们的产物形成速度。

RESEARCH INITIATION AWARD: INVESTIGATION OF THE MECHANISMS RESPONSIBLE FOR INCREASED ANTIBODY PRODUCTION BY HYBRIDOMAS EXPOSED TO STRESS ABSTRACT Monoclonal antibodies are among the largest-volume products made using mammalian cells. Increased antibody production has been reported for hybridomas exposed to environmental stress. The objectives of this project are to characterize the mechanisms responsible for increased antibody production, and to identify reactor conditions with increased productivity. Concurrent changes in energy metabolism and DNA synthesis will be monitored in order to determine the interrelationships between the regulation of cell growth, energy production and protein expression. An understanding of these relationships is required in order to decouple cell growth from product formation. Excessive cell growth is not desired because expensive medium is wasted on the production of biomass. Reduced cell growth rates are also desirable because many primary, hybrid, and genetically- altered cell lines decrease their rate of product formation after repeated cell divisions.