Molecular Genetics of Streptomyces Differentiation

该基金的目的是确定彩链霉菌形态和生理分化的遗传调控方面。重点是从营养菌落到分化菌落的转变，表现为抗生素合成的开始和孢子前空生菌丝的生长。具体目标是：1。新的发育突变体的遗传和表型特征。我们将通过进行互补和精细结构定位研究来完善新发现的absA， bldH位点的遗传特征。我们还将继续对突变体进行表型表征。2. 发育新基因absA、bldG和bldH的克隆及分子特征分析。我们将克隆这些基因，并利用克隆的等位基因进行互补研究，以及对野生型和突变型等位基因进行测序。这种对abs和bld基因的遗传和分子分析不仅有助于我们对微生物多细胞发育和分化的理解，而且还有助于合理操纵自然发生的抗生素合成。

The objective of the grant is to define aspects of the genetic regulation of Streptomyces coelicolor morphological and physiological differentiation. The emphasis is on the transition from the vegetative to the differentiated colony, as manifested by onset of antibiotic synthesis and by the growth of prespore aerial hyphae. The specific aims are: 1. Genetic and phenotypic Characterization of the new developmental mutants. We will refine the genetic characterization of the newly identified absA, bldH loci by performing complementation and fine-structure mapping studies. We will also continue the phenotypic characterization of the bld mutants. 2. Cloning and molecular characterization of the new developmental genes absA, bldG and bldH. We will clone these genes and use the cloned alleles for complementation studies and for sequencing the wild-type and mutant alleles. This genetic and molecular analysis of the abs and bld genes promises to contribute not only to our understanding of microbial multicellular development and differentiation, but also to the potential for rational manipulation of naturally occurring antibiotic synthesis.