Membrane-Associated Phosphorylation

该项目探索了亚硫酸盐替代光激活预还原或胰酶处理的菠菜类囊体潜在ATPase的可能机制。亚硫酸盐可能阻止ADP和镁离子紧密结合的抑制作用。在不同的能量水平下，以及用亚硫酸盐激活类囊体体的不同状态下，研究了镁离子和ADP抑制的动力学。还原的CF1，通过在35%甲醇中的镁三磷酸腺苷水解而耗尽的腺苷酸盐应该是永久有效的。最初的反应将用停流技术来研究，看看滞后是被亚硫酸盐消除还是被腺苷耗尽消除。贾根多夫博士发现，亚硫酸盐可以替代光，在光合作用过程中产生能量。他现在正在利用这种效应来研究叶绿体中能量产生的基本机制。这一过程对地球上的生命至关重要，因为它将光转化为化学能来生产碳水化合物。

This project explores possible mechanisms in the sulfite replacement of light in activating latent ATPase of pre- reduced or trypsin treated spinach thylakoids. Sulfite might prevent inhibition by tightly bound ADP and magnesium ions. Kinetics of Mg2+ and ADP inhibition are being investigated at different levels of energization, and with different states of thylakoid activation using sulfite. Reduced CF1, depleted of adenylates by MgATP hydrolysis in 35% methanol should be permanently active. Initial reactions will be studied by stop-flow technique, to see if lags are eliminated by sulfite or by adenylate depletion. Dr. Jagendorf has made the interesting observation that sulfite can substitute for light leading to energy generation during photosynthesis. He is now using this effect to study the basic mechanism of energy generation in chloroplasts. This process is of utmost importance to life on earth as it converts light to chemical energy for the production of carbohydrates.***//