Standard and Fluorescence Cytophotometry Equipment for the Undergraduate Curriculum and Student Research

生物系在五门高级生物课程和本科生研究项目中发现了一个提高和多样化实验室能力的机会。这一系列目标将通过购置所需的部件来实现，这些部件是建立和完成具有荧光能力的复杂细胞光度测量系统所需的部件；也就是说，新闻部以前已开始用自己的资源组装一个职能单位。增加的实验室活动将包括遗传学的DNA含量测量、细胞周期研究和染色体分析；细胞生物学的细胞化学和荧光显微镜；分子生物学的荧光细胞光度测定和生物素化DNA杂交；微生物学的微生物鉴定；免疫学的间接荧光和直接免疫荧光；以及一些定性和定量技术和额外的本科生研究选择。大量使用这些技术的生物学某些方面的科学进步与课程的性质、教师的专业知识和学生的兴趣非常吻合。接受资助的机构正在将NSF的奖励与从非联邦来源获得的同等金额相匹配。

This Biology Department has identified an opportunity to improve and diversify its laboratory capabilities in five upper division biology courses and in the undergraduate student research program. This entire array of objectives will be met by the acquisition of the components needed to build upon and complete a sophisticated cytophotometer system with fluorescence capabilities; that is, a functional unit which the Department had previously begun to assemble with their own resources. The added laboratory activities will include measurements of DNA content, cell cycle studies, and chromosome analyses in genetics; cytochemistry and fluorescence microscopy in cell biology; fluorescence cytophotometry and biotinylated DNA hybridization in molecular biology; microbe identification in microbiology; indirect fluorescence and direct immunofluorescence in immunology; and a number of qualitative and quantitative techniques and additional undergraduate research options. Scientific advances in certain aspects of biology which heavily utilize these techniques coincide very well with the nature of the curriculum, faculty expertise, and student interest here. The grantee institution is matching the NSF award with an equal sum obtained from non-Federal sources.