Analysis of the Potential for Targeted Host Specificity in Bacterial Plant-Inoculants

通过接种可以改善植物和土壤的健康 植物根部的有益细菌。 特别是， 防治植物真菌病害的细菌接种剂 根已被证明在限制条件下是成功的。 然而，这种方法的潜力还有待开发。 在现场实现。 一个原因是， 细菌产品的范围有限， 由于缺乏建立高水平的能力， 不同作物根际种群数量 不同的环境条件。 这项计划的目标是分离出 提高荧光假单胞菌的能力， 在不同作物的根际 植物 所提出的方法是首先组装一个良好的- 荧光假单胞菌的特征集合， 可以进行分子遗传操作，然后 确定它们的根际能力是否是宿主- 对三种不同的作物都有特异性 成功在于此 这将为根的发展铺平道路， 作为土壤微生物杀虫剂的假单胞菌群 传播的真菌病原体，并提供了一个可行的替代方案， 使用化学药品来控制许多植物病害。

Plant and soil health can be improved through the inoculation of plant roots with beneficial bacterial. In particular, bacterial inoculants that control fungal diseases of plant roots have proven successful under restricted conditions. However, the potential of this approach has yet to be realized in the filed. One reason is that individual bacterial products are limited in their range of applicability due their lack of ability to establish high populations in the rhizosphere of different crops under different environment conditions. The goal of this proposal is to isolate genes that would improve the competence of fluorescent pseudomonads to establish themselves in the rhizosphere of different crop plants. The approach proposed is first to assemble a well- characterized collection of fluorescent pseudomonads that are amenable to molecular genetic manipulation, and then to determine whether their rhizosphere competence is host- specific for three different crop plants. Success is in this approach will pave the way for the development of root- colonizing pseudomonads as microbial pesticides for soil- borne fungal pathogens, and provide a viable alternative to the use of chemicals for the control of many plant diseases.