Purification of Complex Biological Mixtures by Displacement Chromatography: Extension to Group-Specific Affinity Supports and Development of Novel Displacer Compounds

前人关于置换层析同时提纯和浓缩生物分子的工作扩展到：生物分子复杂混合物的分离、新型置换化合物的开发和亲和置换层析的研究。利用前向微量层析获得的吸附数据，选择合适的置换剂和层析条件进行置换实验。用填充了各种固定相材料的分析柱和制备柱进行了置换实验。鉴定和合成了适用于各种色谱系统的新型置换化合物。这项研究大大增加了可用置换剂化合物的数量，并为未来生物分离问题选择合适的置换剂提供了基本指导。基团特定的亲和层析材料，如固定化活性染料、凝集素和金属螯合体系，被研究在置换模式中的潜在用途。在置换模式中使用亲和载体有望显著提高这些选择性层析系统的产量和纯度。这项研究将极大地扩大置换层析的范围，并将促进该技术在生物药物纯化中的广泛应用。

Previous work on the simultaneous purification and concentration of biomolecules by displacement chromatography is expanded to include: separations of complex mixtures of biomolecules, development of novel displacer compounds and an investigation of affinity displacement chromatography. Adsorption data obtained from frontal microscale chromatography is used to select appropriate displacers and chromatographic conditions for the displacement experiments. Displacement experiments are carried out with analytical and preparative columns packed with a variety of stationary phase materials. New classes of displacer compounds are identified and synthesized for various chromatographic systems. This research significantly increases the arsenal of available displacer compounds and provides basic guidelines for the selection of appropriate displacers for future bioseparation problems. Group-specific affinity chromatographic materials such as immobilized reactive dyes, lectins, and metal chelate systems are investigated for their potential use in the displacement mode. The use of affinity supports in the displacement mode is expected to significantly increase the throughput and purity attained with these selective chromatographic systems. This research will significantly expand the scope of displacement chromatography and will facilitate the widespread use of the technique for the purification of biopharmaceuticals.