Metabolic Pathway Responsible for Deuterium Enrichment in CAM Plants

氢同位素掺入过程中分馏的研究 转化为植物生物质可能在植物中具有重要应用 生物化学，生理学，生理生态学和其他一些 领域的 在这个提议中特别感兴趣的是氘 在来自CAM的纤维素的不可交换氢中观察到富集 植物 有证据表明，生物化学反应是 负责氘的富集 的目的 在这项建议中概述的实验是确定哪些代谢 途径负责CAM植物中的氘富集。 整个 植物和组织培养实验在这里提出。 的结果 这些实验将决定是否能进行氘浓缩 在CAM植物中发现的糖酵解-异生周期中。 一个 增加了对氘过程的了解 在CAM植物中的富集将在研究中具有重要意义， 碳水化合物库和源库关系。 更广泛地说， 这项研究将有重要的应用研究， 光合系统与环境参数的关系。

The study of hydrogen isotopic fractionations during its incorporation into plant biomass may have important applications in plant biochemistry, physiology, physiological ecology and several other fields. Of particular interest in this proposal is the deuterium enrichment observed in non-exchangeable hydrogens of cellulose from CAM plants. Evidence is presented showing that biochemical reactions are responsible for this deuterium enrichment. The objective of the experiments outlined in this proposal is to determine which metabolic pathway is responsible for deuterium enrichment in CAM plants. Whole plant and tissue culture experiments are proposed here. The results of these experiments will determine whether deuterium enrichment can occur during the glycolysis-gluconeogenesis cycle found in CAM plants. An increased knowledge of the processes responsible for deuterium enrichment in CAM plants will be significant in the study of carbohydrate pools and source sink relations. More broadly, the results of this study will have important applications in the study of photosynthetic systems in relation to environmental parameters.