New Techniques for Affinity Precipitation of Proteins

本项目旨在开发两种从复杂介质中纯化酶和抗体等生物大分子的新技术，同时达到高选择性、高产量和高活性。该技术属于亲和分离技术，但其特点是使用亲和表面活性剂。建议使用亲和表面活性剂通过限制困扰其他蛋白质沉淀技术的不同蛋白质之间的非特异性疏水相互作用来提高有效性。该研究的靶蛋白是寡聚脱氢酶和一类IgG抗体。作为项目的一部分，亲和表面活性剂将被合成和纯化。表面活性剂可以直接溶解在蛋白质水溶液中，也可以掺入单层囊泡中。​沉淀是许多化工过程中重要的净化过程。由于蛋白质之间疏水相互作用的非特异性，它在生物技术中的应用有限。亲和表面活性剂沉淀蛋白质是提高沉淀技术在生物技术下游加工中的应用前景广阔的技术。

This project aims to develop two new techniques for purifying biological macromolecules such as enzymes and antibodies from complex media while attaining high selectivity, yield, and activity. The techniques belong to the class of affinity separation techniques but are distinguished by using affinity surfactants. It is proposed that the use of affinity surfactants increases effectiveness by limiting nonspecific hydrophobic interactions between different proteins that plague other protein precipitation techniques. The target proteins for the study are the oligomeric dehydrogenase enzymes and the class of IgG antibodies. Affinity surfactants will be synthesized and purified as part of the project. The surfactants will be used either directly dissolved in the aqueous protein solution or by incorporation into unilamellar vesicles. Precipitation is an important purification process in many chemical processes. It has had limited utility in biotechnology because of the nonspecific nature of hydrophobic interactions between proteins. Affinity surfactant precipitation of proteins is a promising technique to improve the usefulness of precipitation technology in downstream processing in biotechnology.