Capillary Gas Chromatograph-Mass Spectrometer for BiologicalChemistry

请拨经费购买一台专用毛细管气相色谱-质谱仪(CGC-MS)仪器，以促进由五个一级用户组和五个二级用户组进行的生物化学研究。挥发物的复杂混合物将通过毛细管气相色谱分离，通过化学电离或电子撞击进行电离，并由计算机驱动的采集系统进行分析。目前，校内外都没有能够提供这种实际分析能力的仪器。该仪器将在几个主要项目中使用。1.分析蛾的信息素代谢，鉴定保幼激素，从昆虫组织中分离新的幼虫和抗幼虫，并分析含氟和含硅的角鲨烯类似物的代谢物。2.用P-450模型从催化反应中分离同位素标记化合物。3.检测盘基网柄菌耐药突变体膜类固醇成分的变化。4.在逆境植物中羽扇豆碱的鉴定。由五个小用户提出的项目包括：1.分析过氧化物酶作用得到的酚类代谢物；2.闪速真空热解分析非挥发性分子；3.分子识别实验中复杂杂环的分析；4.不对称合成新方法的开发；5.金刚烷基衍生物的面部立体化学分析。

Funding is requested for the purchase of a dedicated capillary gas chromatograph-mass spectrometry (CGC-MS) instrument to facilitate research in biological chemistry conducted by five primary and five secondary user groups. Complex mixtures of volatiles would be separated by capillary GC, ionized by chemical ionization or electron impact, and analyzed by a computer-driven acquisition system. No current instrumentation is available on or off campus which could provide this hands-on analytical capability. The instrument will be used in several major projects. 1. analysis of pheromone metabolism in moths, for identification of juvenile hormones, for isolation of novel juvenoids and antijuvenoids from insect tissues, and for analysis of metabolites of fluorine-and silicon-containing squalene analogs. 2. separation of isotopically-labeled compounds from catalytic reactions with P-450 models. 3. to examine changes in membrane steroid composition which occur in antibiotic-resistant mutants of Dictyostelium discoideum. 4. in the identification of lupine alkaloids in stressed plants. Projects proposed by five minor users include 1. analysis of phenolic metabolites obtained from peroxidase action; 2. flash vacuum pyrolysis for analysis of nonvolatile molecules; 3. analysis of complex heterocycles in molecular recognition experiments; 4. development of new synthetic methods in asymmetric synthesis; and 5. analysis of facial stereochemistry in adamantyl derivatives.