Conference on Regulation of Extravascular Fibrinolysis in Nervous System Development and Disease; July 2-8, 1989; Acqua fredda di Maratea, Italy

在过去的十年里，血栓形成和纤溶的研究出现了戏剧性的爆炸性增长。这些过程涉及作用于前体酵素的酶的级联作用，从而允许多水平的翻译后调节。调节也发生在转译和翻译水平上，以及这些蛋白水解酶识别和切割的精确键上。翻译后调节也由特定的抑制物发生，蛇具有精确的活性部位，受激素、调节剂和其他分子的调节。凝血酶和纤溶酶原激活物(PA)的细胞表面受体已经被发现，允许另一个维度的调节。这些分子在细胞迁移和组织重塑中的作用正在扩大。在转移过程中，炎症和生理过程如排卵、蛋白水解酶和抑制物等正在被积极研究。最近的信息表明，它在神经系统中扮演着重要角色。此时，有必要将这一新兴领域的领导者聚集在一起，就神经系统的发育、维持、可塑性和退化等令人感兴趣的分子交流仍处于萌芽状态的知识。这个高级研究研讨会的关键是“血管外”这一术语。这些分子是神经元和神经胶质细胞的基因产物。本研讨会所依据的证据有四个：(1)神经生长锥顶端存在和释放尿激酶(UPA)和组织纤溶酶原激活物(TPA)，(2)神经胶质源性连接蛋白和蛋白连接蛋白(PNI)的同一性，(3)PNI在神经肌肉突触的定位，(4)检测从阿尔茨海默病患者神经性斑块中克隆的B-淀粉样蛋白中的丝氨酸蛋白和昆尼茨抑制物序列。

The last decade has seen a dramatic explosion in the studies of thrombogenesis and fibrinolysis. These processes involve cascades of enzymes acting on precursor zymogens, thus allowing for multi-level, post-translational regulation. Regulation also occurs at the transciptional and translational levels and in the precise bonds which these proteolytic enzymes recognize and cleave. Post-translational regulation also occurs by specific inhibitors, the serpins, with precise active sites, regulated by hormones, modulators, and other molecules. Cell surface receptors for thrombin and the plasminogen activators (PAs) have been found, allowing for another dimension for regulation. Roles for these molecules in cell migration and tissue remodelling are expanding. In metastasis, inflammation and physiological processes such as ovulation, proteases and inhibitors are being actively studied. Recent information suggests important roles in the nervous systems. At this time, it is necessary to bring leaders in this emerging field together to communicate the still nascent state of knowledge about such intriguing molecules in the development, maintenance, plasticity and degeneration of the nervous system. The key for this advanced research workshop is the term "extravascular." These molecules are gene products of neuron and glia cells. The evidence on which this workshop is based is four-fold: (1) presence and release of urokinase (uPA) and tissue PA (tPA) at tips of nerve growth cones, (2) identity of glia-derived nexin and protease nexin (PNI), (3) localization of PNI at neuromuscular synapses, (4) detection of serpin and Kunitz inhibitor sequences in B-amyloid cloned from neuritic plaques of victims of Alzheimer's disease.