Process Control in Solid Substrate Fermentation

本研究旨在建立一种基于蒸发冷却和水分替代策略的温湿度控制系统，以调节少孢根霉在膨化玉米上固体基质发酵的生物量生产力。过程控制方案基于自适应控制算法。控制系统将与生物量浓度和基质转化的在线测量值相结合，这些值包括产热、耗氧、二氧化碳产生和水平衡。测量值和估计值将通过数学建模相关联。丝状真菌固体底物发酵（SSF）是一种可行的替代深层发酵，如果该过程可以扩大到工业规模，如果关键发酵参数可以在线测量过程控制的目的。固体底物发酵的主要问题是调节发酵罐内的温度和湿度，使其生长条件达到最佳。建议通过结合蒸发冷却和由复杂的“智能”控制器指导的水添加来做到这一点。工艺条件也要用数学模型来估计。

This research is aimed at developing a temperature and moisture control system based on evaporative cooling and water replacement strategy in order to regulate the biomass productivity in the solid substrate fermentation of Rhizospus oligosporus on extruded corn. The process control scheme is based on adaptive control algorithm. The control system will be coupled to the estimation of biomass concentration and substrate conversion from on-line measured values of heat generation, oxygen consumption, carbon dioxide production, and water balance. Measured and estimated values will be correlated through mathematical modelling. Solid substrate fermentation (SSF) with filamentous fungi is a viable alternative to submerged fermentation if the process could be scaled up to industrial size and if the critical fermentation parameters can be measured on-line for process control purposes. The main problem in solid substrate fermentation is to regulated the temperature and the humidity in the fermenter so that the growth condition is optimized. It is proposed to do this by combining evaporative cooling and water addition as directed by a sophisticated "smart" controller. Process conditions are also to be estimated by mathematical models.