Molecular Characterization of the Enzymatic Machinery Involved in Antibody Gene Recombination

本研究旨在进一步研究抗原受体基因重组所涉及的酶机制。这一领域的研究大多局限于DNA水平上对重组事件的研究。该项目将建立一个体外重组系统，以进一步剖析导致形成完整V-(D)-J结构的分子事件。这样的系统对于V-(D)-J重组酶可能组成的酶学特性是必不可少的。在计划期间，PI将进行必要的初步实验，以制定适当的策略来建立这样的体外重组系统。由于在这一特定领域的研究总体上缺乏，该研究所将有必要在相关领域进行广泛的文献搜索，以寻找可能使拟议的项目更可行的酶分析和反应条件。

This research project is to further characterize the enzymatic machinery involved in antigen receptor gene recombination. The research in this field has been mostly limited to studies of recombination events at the DNA level. This project will establish an in vitro recombination system to further dissect the molecular events leading to the formation of a complete V-(D)-J structure. Such a system will be essential for the enzymatic characterization of the putative components of the V-(D)-J recombinase. During the planning period the PI will conduct the necessary preliminary experiments to formulate the appropriate strategies to establish such an in vitro recombination system. Due to the overall lack of research in this particular area, it will be necessary for the PI to conduct an extensive literature search in related fields for enzymatic assays and reaction conditions which might make the proposed project more feasible.